CtIP antibody (mAb) (Clone 14-1)

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

ChIP, ICC, IF, IP, WB

CloneNo.

14-1

Cat No : 61141,61142,61942

Synonyms

CtIP, CTBP interacting protein, RBBP8, RIM, COM1, transcriptional regulator, tumor suppressor, C-Terminal Binding Protein, Rb, repressor, TFIID, TFIIB, p130, Ikaros, DNA, brca1, cancer, monoclonal, clone 14-1, sample

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Validation Data Gallery

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  CtIP antibody mpAb) tested by Western blot 20 ug of HEK293 whole cell extract was run on SDS-PAGE and probed with antibody at 1 ug/ml.

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Product Information

• Tested Applications: ChIP, ICC, IF, IP, WB

  Applications Validated by Active Motif: WB: 1 - 2 ug/ml

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: This CtIP antibody was raised against a recombinant protein corresponding to amino acid residues 620-897 of human CtIP.
• Full Name: CtIP antibody (mAb) (Clone 14-1)
• Synonyms: CtIP, CTBP interacting protein, RBBP8, RIM, COM1, transcriptional regulator, tumor suppressor, C-Terminal Binding Protein, Rb, repressor, TFIID, TFIIB, p130, Ikaros, DNA, brca1, cancer, monoclonal, clone 14-1, sample
• Molecular weight: 120 kDa
• GenBank accession number: NP_002885
• RRID: AB_2714164
• Purification Method: Protein A Chromatography
• Buffer: Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
• Storage: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

CtIP (CTBP interacting protein, RBBP8, RIM, COM1) is a transcriptional regulator and tumor suppressor first identified through its interaction with CTBP (C-Terminal Binding Protein). CtIP binds to the Rb protein and serves as a transcriptional repressor, through recruitment of CtBP and also by interaction with general transcription factors TFIID and TFIIB. CtIP also interacts with Rb-related protein p130 and Ikaros, a master regulator in lymphocyte development. CtIP is phosphorylated upon DNA damage (likely by ATM and/or ATR), interacts with BRCA1 and plays a critical role in facilitating DNA damage repair. Mutations that abolish the interaction between CtIP and BRCA1 result in loss of cell cycle control leading to the development of cancer.