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Chd5 antibody (mAb) (Clone 5A10)

Host / Isotype

Rat / IgG2a

Reactivity

Human, Mouse

Applications

ICC, IF, WB

CloneNo.

5A10

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Cat No : 61683,61684 61683

Synonyms

Chd5, Chromodomain Helicase DNA Binding Protein 5, EC 3.6.4.12, CHD-5, KIAA0444, ATP-Dependent Helicase CHD5, EC 3.6.1, Chromodomain-Helicase-DNA-Binding Protein 5, mouse, neuroblastoma, immunocytochemistry, icc, immunofluorescence, if, western blotting, wb, antibody, antibodies, monoclonal, mab, sample

Validation Data Gallery

  • Chd5 antibody (mAb) (Clone 5A10) tested by immunofluorescence. Left: HeLa cells. Right: E12.5 mouse gut. Both stained with Chd5 antibody.

    Chd5 antibody (mAb) (Clone 5A10) tested by immunofluorescence. Left: HeLa cells. Right: E12.5 mouse gut. Both stained with Chd5 antibody.

  • Chd5 antibody (mAb) (Clone 5A10) tested by Western blot. Chd5 antibody detection by Western blot. The analysis was performed using 40 ug of HeLa nuclear extract and Chd5 antibody at a 2 ug/ml dilution.

    Chd5 antibody (mAb) (Clone 5A10) tested by Western blot. Chd5 antibody detection by Western blot. The analysis was performed using 40 ug of HeLa nuclear extract and Chd5 antibody at a 2 ug/ml dilution.

Product Information

Tested Applications ICC, IF, WB

Applications Validated by Active Motif: ICC/IF: 2 - 5 ug/ml dilution WB*: 1 - 2 ug/ml dilution The addition of 0.1% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required. *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.
Tested Reactivity Human, Mouse
Host / Isotype Rat / IgG2a
Class Monoclonal
Type Antibody
Immunogen This antibody was raised against a peptide within the C-terminal region of mouse Chd5.
Full Name Chd5 antibody (mAb) (Clone 5A10)
Synonyms Chd5, Chromodomain Helicase DNA Binding Protein 5, EC 3.6.4.12, CHD-5, KIAA0444, ATP-Dependent Helicase CHD5, EC 3.6.1, Chromodomain-Helicase-DNA-Binding Protein 5, mouse, neuroblastoma, immunocytochemistry, icc, immunofluorescence, if, western blotting, wb, antibody, antibodies, monoclonal, mab, sample
Molecular weight 260 kDa
GenBank accession numberNP_001074845
RRIDAB_2793734
Purification Method Protein G Chromatography
Buffer Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

Chd5 (Chromodomain Helicase DNA Binding Protein 5) is member of the CHD subfamily of chromatin remodeling SWI/SNF proteins, containing chromodomains and a SNF2-like ATPase/helicase domain, that play a role in chromatin remodeling and gene transcription. Chd5 is also a potential tumor suppressor that may play a role in neuronal development.