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Caspase-7 antibody (mAb) (Clone 25B204.1)

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB

CloneNo.

25B204.1

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Cat No : 40929 40929

Synonyms

Caspase-7, Caspase7, Caspase 7, mAb, monoclonal, CASP7, apoptosis-related cysteine peptidase 7, antibody, antibodies

Validation Data Gallery

  • Caspase-7 mAb tested by Western blot. Caspase-7 detection by Western blot. The analysis was performed using Jurkat nuclear extracts and Caspase-7 mAb at dilutions of 2 ug/ml (lane 1) and 0.5 ug/ml (lane 2). This antibody only detects the 35 kDa caspase-7 corresponding to pro-caspase-7 in non-apoptotic Jurkat cells.

    Caspase-7 mAb tested by Western blot. Caspase-7 detection by Western blot. The analysis was performed using Jurkat nuclear extracts and Caspase-7 mAb at dilutions of 2 ug/ml (lane 1) and 0.5 ug/ml (lane 2). This antibody only detects the 35 kDa caspase-7 corresponding to pro-caspase-7 in non-apoptotic Jurkat cells.

Product Information

Tested Applications WB

Applications Validated by Active Motif: WB: 1 - 2 ug/ml dilution For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all blocking solutions may also reduce background. Individual optimization may be required.
Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen This Caspase-7 antibody was raised against a recombinant human Caspase-7 protein.
Full Name Caspase-7 antibody (mAb) (Clone 25B204.1)
Synonyms Caspase-7, Caspase7, Caspase 7, mAb, monoclonal, CASP7, apoptosis-related cysteine peptidase 7, antibody, antibodies
Molecular weight 35 kDa
GenBank accession numberNP_001218
RRIDAB_2793444
Purification Method Affinity Purified
Buffer PBS containing 0.02% sodium azide. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

Caspase-7 (CASP7 or apoptosis-related cysteine peptidase 7) is involved in the activation cascade of caspases responsible for apoptosis execution. The precursor form of Caspase-7 is cleaved by Caspase-3 and Caspase-10, which is activated by cell death stimuli and induces apoptosis. Caspase-7 cleaves and activates sterol regulatory element binding proteins (SREBPs) and proteolytically cleaves poly(ADP-ribose) polymerase (PARP, see PARP-1 C-terminal and PARP-1 N-terminal antibodies).