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CTBP2 antibody (pAb)

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

ChIP, ChIP-Seq, WB

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Cat No : 61261,61262 61261

Synonyms

CTBP2, C-terminal binding protein 2, ribeye, pAb, polyclonal, rabbit, trancription, sample

Validation Data Gallery

  • CTBP2 antibody (pAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit (Cat. No. 53040) with 30 ug of chromatin from mouse liver and 10 ul of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 15 million sequence tags were mapped to identify CTBP2 binding sites. The image shows binding across a regions of chromosome 11. You can view the complete data set in the UCSC Genome Browser

    CTBP2 antibody (pAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit (Cat. No. 53040) with 30 ug of chromatin from mouse liver and 10 ul of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 15 million sequence tags were mapped to identify CTBP2 binding sites. The image shows binding across a regions of chromosome 11. You can view the complete data set in the UCSC Genome Browser

  • CTBP2 antibody (pAb) tested by Western blot Nuclear extract of HeLa cells (10 ug) probed with CTBP2 antibody (1:1000).

    CTBP2 antibody (pAb) tested by Western blot Nuclear extract of HeLa cells (10 ug) probed with CTBP2 antibody (1:1000).

Product Information

Tested Applications ChIP, ChIP-Seq, WB

Applications Validated by Active Motif: ChIP-Seq: 4 ug each ChIP: 10 ul per ChIP WB*: 1:500 - 1:1,000 dilution *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot.
Tested Reactivity Human, Mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen This CTBP2 antibody was raised against a peptide in the C-terminal region of human CTBP2. This antibody recognizes both isoform 1 and 2.
Full Name CTBP2 antibody (pAb)
Synonyms CTBP2, C-terminal binding protein 2, ribeye, pAb, polyclonal, rabbit, trancription, sample
Molecular weight 50 kDa
GenBank accession numberNP_001077383
RRIDAB_2793573
Purification Method Affinity Purified
Buffer Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

The CTBP2 (C-terminal binding protein 2) gene encodes a protein that is a corepressor targeting diverse transcription regulators. It functions in brown adipose tissue (BAT) differentiation. Isoform 2 probably acts as a scaffold for specialized synapses. Isoform 2 is also phosphorylated upon DNA damage, probably by ATM or ATR at Thr179; Ser181 and Ser185. Phosphorylation by HIPK2 on Ser428 induces proteasomal degradation.