CID antibody (mAb) (Clone 4F8)
Host / Isotype
Rat / IgG2a
Reactivity
Drosophila
Applications
ICC, IF
CloneNo.
4F8
Cat No : 61735,61736 61735
Synonyms
Validation Data Gallery
Product Information
| Tested Applications | ICC, IF |
| Tested Reactivity | Drosophila |
| Host / Isotype | Rat / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | This antibody was raised against a recombinant protein corresponding to the N-terminus of drosophila CID. |
| Full Name | CID antibody (mAb) (Clone 4F8) |
| Synonyms | CenH3, cid, BcDNA:RE21270, cenH3, CenH3/CID, CenH3[Cid], CenH3[CID], Cenp-A, CENP-A, CENP-A/Cid, CENP-A/CID, CENP-C, cenpA, CenpA, CENPA, CenpA/CID, CG13329, Cid, CID/CENP-A, Cid[Mel], Dmel\CG13329, drosophila, antibody, antibodies, monoclonal, mab, immunocytochemistry, icc, immunofluorescence, if, sample |
| Molecular weight | 35 kDa |
| GenBank accession number | NP_523730 |
| RRID | AB_2793749 |
| Purification Method | Protein G Chromatography |
| Buffer | Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| Storage | Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage. |
Background Information
CID (Centromer Identifier) is the Drosophila histone H3 variant which replaces canonical H3 in centromeric chromatin at the inner plate of the kinetochore (similar to mammalian CENP-A). CID is required for recruitment and assembly of kinetochore proteins, mitotic progression and chromosome segregation. It may serve as an epigenetic mark that propagates centromere identity through DNA replication and cell division.