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CENP-B antibody (pAb)

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

ICC, IF, IP, WB

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Cat No : 61287,61288 61287

Synonyms

CENP-B, Centromere Protein B, 80kDa, CENP-B, Centromere Protein B (80kD), Centromere Autoantigen B, Centromere Protein B, antibody, antibodies, polyclonal, pab, immunopreciptiation, ip, western blotting, wb, sample

Validation Data Gallery

  • CENP-B antibody (pAb) tested by Immunoprecipitation. 10 ul of CENP-B antibody was used to immunoprecipitate CENP-B from 250 ug of HeLa nuclear cell extract (lane 2). 10 ul of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the CENP-B antibody at a dilution of 1:500.

    CENP-B antibody (pAb) tested by Immunoprecipitation. 10 ul of CENP-B antibody was used to immunoprecipitate CENP-B from 250 ug of HeLa nuclear cell extract (lane 2). 10 ul of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the CENP-B antibody at a dilution of 1:500.

  • CENP-B antibody (pAb) tested by Western blot. CENP-B detection by Western blot. The analysis was performed using 30 ug HeLa nuclear cell extract and CENP-B at a 1:500 dilution.

    CENP-B antibody (pAb) tested by Western blot. CENP-B detection by Western blot. The analysis was performed using 30 ug HeLa nuclear cell extract and CENP-B at a 1:500 dilution.

Product Information

Tested Applications ICC, IF, IP, WB

Applications Validated by Active Motif: IP: 10 ul per IP WB*: 1:500 - 1:2,000 dilution *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot.
Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen This CENP-B antibody was raised against a recombinant protein corresponding to the C-terminal region of human CENP-B.
Full Name CENP-B antibody (pAb)
Synonyms CENP-B, Centromere Protein B, 80kDa, CENP-B, Centromere Protein B (80kD), Centromere Autoantigen B, Centromere Protein B, antibody, antibodies, polyclonal, pab, immunopreciptiation, ip, western blotting, wb, sample
Molecular weight 80 kDa
GenBank accession numberNP_001801
RRIDAB_2793582
Purification Method Affinity Purified
Buffer Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

CENP-B (Centromere protein B) is a protein associated with centromeric DNA and crucial to the formation of the special chromatin structure on the a-satellite DNA repeats of the human centromere. CENP-B specifically binds a 17 bp sequence which may function as a cis-element for centromere-specific nucleosome assembly. CENP-B plays a role in the organization of centromere satellite DNA into a higher order structure, directing centromere formation and kinetochore assembly in mammalian chromosomes.