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CENP-B antibody (pAb)

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

ICC, IF

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Cat No : 61183,61184 61183

Synonyms

CENP-B, Centromere protein B, CENPB, centromeric DNA, kinetochore assembly, sample

Validation Data Gallery

  • CENP-B antibody (pAb) tested by immunofluorescence. Staining of HeLa cells with CENP-B pAb at a 1 ug/ml dilution (green) and a-tubulin (red, Catalog No. 39527) at 1 ug/ml.

    CENP-B antibody (pAb) tested by immunofluorescence. Staining of HeLa cells with CENP-B pAb at a 1 ug/ml dilution (green) and a-tubulin (red, Catalog No. 39527) at 1 ug/ml.

Product Information

Tested Applications ICC, IF

Applications Validated by Active Motif: ICC/IF: 1 - 2 ug/ml dilution
Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen This CENP-B antibody was raised against a recombinant protein corresponding to the C-terminal region of human CENP-B.
Full Name CENP-B antibody (pAb)
Synonyms CENP-B, Centromere protein B, CENPB, centromeric DNA, kinetochore assembly, sample
Molecular weight 67 kDa
GenBank accession numberNP_001801
RRIDAB_2793542
Purification Method Protein A Chromatography
Buffer Purified rabbit IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

CENP-B (Centromere protein B) is a protein associated with centromeric DNA and crucial to the formation of the special chromatin structure on the a-satellite DNA repeats of the human centromere. CENP-B specifically binds a 17 bp sequence which may function as a cis-element for centromere-specific nucleosome assembly. CENP-B plays a role in the organization of centromere satellite DNA into a higher order structure, directing centromere formation and kinetochore assembly in mammalian chromosomes.