CENP-A phospho Ser18 antibody (pAb)

Host / Isotype

Rabbit / IgG

Reactivity

Human, Wide Range Predicted

Applications

DB, ICC, IF, WB

Cat No : 61483,61484

Synonyms

CENPA phospho Ser18, CENPA, phospho, phosphorylation, ser18, serine 18, Centromere Protein A, Centromere Autoantigen A, CENP-A, Centromere Protein A (17kD), CenH3, Centromere-Specific Histone, Centromere Protein A, 17kDa, Histone H3-Like Centromeric Protein A, histone, h3, antibody, pAb, polyclonal, western blot, wb, immunofluorescence, if, dot blot, db, sample

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Validation Data Gallery

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  CENP-A phospho Ser18 antibody (pAb) tested by immunofluorescence. Detection of CENP-A phospho Ser18 by immunofluorescence. HeLa cells were stained with CENP-A phospho Ser18 antibody at a 1:100 dilution. Green: CENP-A phospho Ser18 antibody (pAb) Blue: DAPI

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  CENP-A phospho Ser18 antibody (pAb) tested by Western blot. Top: Recombinant CENP-A (20 ng) was probed with CENP-A phospho Ser18 antibody (1:100 dilution) (A) or with anti-centromere antibody (ACA) (B). CENP-A phospho Ser18 antibody does not detect recombinant CENP-A. Bottom: HeLa nuclear extract (20 ug) was probed with CENP-A phospho Ser18 antibody (1:250 dilution). Lane 1: CENP-A phospho-Ser18 antibody. Lane 2: anti-centromere antibody (ACA).

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  CENP-A phospho Ser18 antibody (pAb) tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of CENP-A phospho Ser18 antibody. Peptides corresponding to the immunogen and related proteins were spotted onto PVDF and probed with the antibody at 1:750. The amount of peptide (picomoles) spotted is indicated next to each row. Lane 1: CENP-A phospho Ser 16 and phospho Ser18. Lane 2: CENP-A unmodified. Lane 3: CENP-A phospho Ser 16. Lane 4: CENP-A phospho Ser 18.

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Product Information

• Tested Applications: DB, ICC, IF, WB

  Applications Validated by Active Motif: ICC/IF: 1:100 dilution WB: 1:100 - 1:500 dilution The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required.

• Tested Reactivity: Human, Wide Range Predicted
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Modification: Phosphorylated
• Immunogen: This antibody was raised against a synthetic peptide containing phosphorylated serine 16 and 18 of human CENP-A.
• Full Name: CENP-A phospho Ser18 antibody (pAb)
• Synonyms: CENPA phospho Ser18, CENPA, phospho, phosphorylation, ser18, serine 18, Centromere Protein A, Centromere Autoantigen A, CENP-A, Centromere Protein A (17kD), CenH3, Centromere-Specific Histone, Centromere Protein A, 17kDa, Histone H3-Like Centromeric Protein A, histone, h3, antibody, pAb, polyclonal, western blot, wb, immunofluorescence, if, dot blot, db, sample
• Molecular weight: 16 kDa
• GenBank accession number: NP_001800
• RRID: AB_2793655
• Purification Method: Affinity Purified
• Buffer: Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
• Storage: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

CENP-A (Centromere Protein A) is a Histone H3-like variant which exclusively replaces conventional H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore. It is required for recruitment and assembly of kinetochore proteins, mitotic progression and chromosome segregation. Recently, phosphorylation of CENP-A on Ser16 and Ser18 was reported and found to be important for chromosome segregation during mitosis.