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Bcl10 antibody (mAb) (Clone 151)

Host / Isotype

Mouse / IgG

Reactivity

Human

Applications

IP, WB

CloneNo.

151

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Cat No : 39393,39394 39393

Synonyms

NFkB, BCL, bcl10, Clone 151, mAb, monoclonal, B-cell CLL/lymphoma 10, antibody, antibodies, sample

Validation Data Gallery

  • Bcl10 antibody (mAb) (Clone 151) tested by Immunoprecipitation. 10 ug of Bcl10 antibody was used to immunoprecipitate Bcl10 from 500 ug of K562 whole cell extract (lane 2). 10 ug of mouse IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the Bcl10 antibody at a 1 ug/ml dilution.

    Bcl10 antibody (mAb) (Clone 151) tested by Immunoprecipitation. 10 ug of Bcl10 antibody was used to immunoprecipitate Bcl10 from 500 ug of K562 whole cell extract (lane 2). 10 ug of mouse IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the Bcl10 antibody at a 1 ug/ml dilution.

  • Bcl10 antibody (mAb) (Clone 151) tested by Western blot. Bcl10 detection by Western blot. The analysis was performed using 20 ug HeLa nuclear extract and Bcl10 mAb (Clone 151) at a 1 ug/ml dilution.

    Bcl10 antibody (mAb) (Clone 151) tested by Western blot. Bcl10 detection by Western blot. The analysis was performed using 20 ug HeLa nuclear extract and Bcl10 mAb (Clone 151) at a 1 ug/ml dilution.

Product Information

Tested Applications IP, WB

Applications Validated by Active Motif: IP: 10 ug per IP WB: 0.5 - 2 ug/ml dilution
Tested Reactivity Human
Host / Isotype Mouse / IgG
Class Monoclonal
Type Antibody
Immunogen This Bcl10 antibody was raised against full-length recombinant human Bcl10 protein.
Full Name Bcl10 antibody (mAb) (Clone 151)
Synonyms NFkB, BCL, bcl10, Clone 151, mAb, monoclonal, B-cell CLL/lymphoma 10, antibody, antibodies, sample
Molecular weight 33 kDa
GenBank accession numberNP_003912
RRIDAB_2615055
Purification Method Protein G Chromatography
Buffer Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

Bcl10 (B-cell CLL/lymphoma 10) protein promotes apoptosis and activation of NFkB (NFkB p50 & NFkB p65) via NIK and IKK (IKKa, IKKb, IKKi/IKKe and IKKg). Bcl10 protein contains a caspase recruitment domain (CARD) which interacts with other CARD domain containing proteins to regulate NFkB signaling. It is also a substrate for MALT1.