BRD8 / SMAP2 antibody (pAb)

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

ChIP, ChIP-Seq, WB

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Cat No : 61007,61008 61007

Synonyms

BRD8, Bromodomain-containing protein 8, SMAP2, TRCP120, Bromodomain, chromatin, NuA4 histone acetyltransferase complex, acetylated histones, BRD, transcriptional activation, bromo, sample



Product Information

Tested Applications ChIP, ChIP-Seq, WB

Applications Validated by Active Motif: ChIP: 10 ul per ChIP ChIP-Seq:10 ul each WB*: 1:500 - 1:1,000 dilution ChIP-Seq validation was performed by Active Motif's Epigenetics Services; the complete data set is available in the UCSC Genome Browser by clicking here. *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen This BRD8 / SMAP2 antibody was raised against a peptide corresponding to amino acids within the N-terminal region of human BRD8.
Full Name BRD8 / SMAP2 antibody (pAb)
Synonyms BRD8, Bromodomain-containing protein 8, SMAP2, TRCP120, Bromodomain, chromatin, NuA4 histone acetyltransferase complex, acetylated histones, BRD, transcriptional activation, bromo, sample
Molecular weight 145 kDa
GenBank accession numberNP_006687
RRIDAB_2615053
Purification Method Affinity Purified
Buffer Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

BRD8 (Bromodomain-containing protein 8, SMAP2, TRCP120) is a member of the Bromodomain-containing family of chromatin associated proteins. BRD8 is a member of the NuA4 histone acetyltransferase complex, involved in the transcriptional activation of specific genes through the acetylation of histones H2A and H4. BRD8 contains two Bromodomains, a motif that has been demonstrated to facilitate interaction with acetylated histones.