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BRD3 antibody (pAb)

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

ChIP, ChIP-Seq, WB

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Cat No : 61489,61490 61489

Synonyms

BRD3, Bromodomain Containing 3, RING3L, RING3-Like Protein, ORFX, Bromodomain-Containing Protein 3, Bromodomain-Containing 3, transcription regulation, chromatin, gata1, antibody, polyclonal, pAb, western blotting, wb, sample

Validation Data Gallery

  • BRD3 antibody (pAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit (Cat. No. 53040) with 30 ug of chromatin from human H526 cells and 10 ul of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 16 million sequence tags were mapped to show the expected promoter localization.

    BRD3 antibody (pAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit (Cat. No. 53040) with 30 ug of chromatin from human H526 cells and 10 ul of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 16 million sequence tags were mapped to show the expected promoter localization.

  • BRD3 antibody (pAb) tested by ChIP. Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit (Cat. No. 53040) with 30 ug of chromatin from human H526 cells and 10 ul BRD3 antibody. ChIP DNA was used in qPCR with the control primer or gene-specific primer as indicated. Data are presented as Binding Events Detected per 1000 Cells using Active Motif's Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction.

    BRD3 antibody (pAb) tested by ChIP. Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit (Cat. No. 53040) with 30 ug of chromatin from human H526 cells and 10 ul BRD3 antibody. ChIP DNA was used in qPCR with the control primer or gene-specific primer as indicated. Data are presented as Binding Events Detected per 1000 Cells using Active Motif's Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction.

  • BRD3 antibody (pAb) tested by Western blot. Detection of BRD3 by Western blot analysis. Lane 1: Whole cell extract of HeLa cells (20 ug). Lane 2: Whole cell extract of NIH-3T3 cells. Both probed with BRD3 antibody (pAb) at a 1:500 dilution.

    BRD3 antibody (pAb) tested by Western blot. Detection of BRD3 by Western blot analysis. Lane 1: Whole cell extract of HeLa cells (20 ug). Lane 2: Whole cell extract of NIH-3T3 cells. Both probed with BRD3 antibody (pAb) at a 1:500 dilution.

Product Information

Tested Applications ChIP, ChIP-Seq, WB

Applications Validated by Active Motif: ChIP: 10 ul per ChIP ChIP-Seq & ChIP-chip: 10 ul each WB: 1:500- 1:2,000 dilution
Tested Reactivity Human, Mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen This antibody was raised against a peptide within the C-terminal region of mouse BRD3.
Full Name BRD3 antibody (pAb)
Synonyms BRD3, Bromodomain Containing 3, RING3L, RING3-Like Protein, ORFX, Bromodomain-Containing Protein 3, Bromodomain-Containing 3, transcription regulation, chromatin, gata1, antibody, polyclonal, pAb, western blotting, wb, sample
Molecular weight 100 kDa
GenBank accession numberNP_001107046
RRIDAB_2737028
Purification Method Affinity Purified
Buffer Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

BRD3 (Bromodomain Containing 3) is a member of the mammalian BET (Bromodomain Extra Terminal) family of proteins, binds hyperacetylated chromatin and has been reported to interact with acetylated Histone H4K5, Histone H4K12 and Histone H3K14. It plays a role in the regulation of transcription, probably by chromatin remodeling and interaction with transcription factors. Regulates transcription by promoting the binding of the transcription factor GATA1 to its targets (By similarity). Regulates transcription of the CCND1 gene.