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BRD2 antibody (pAb)

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

ChIP, ChIP-Seq

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Cat No : 61797,61798 61797

Synonyms

Bromodomain, BRD3, BRD4, BRD, chromodomain, Tudor domain, chromatin, histone, polyclonal antibody, chip, chip-seq, epigenetics

Validation Data Gallery

  • ChIP-Seq of BRD2 antibody (pAb). ChIP was performed using BRD2 polyclonal antibody with 30 ug chromatin from the DHL4 cell line and 6 ul of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 16 million sequence tags were mapped to identify BRD2 binding sites. The image shows binding across a region of chromosome 1.

    ChIP-Seq of BRD2 antibody (pAb). ChIP was performed using BRD2 polyclonal antibody with 30 ug chromatin from the DHL4 cell line and 6 ul of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 16 million sequence tags were mapped to identify BRD2 binding sites. The image shows binding across a region of chromosome 1.

Product Information

Tested Applications ChIP, ChIP-Seq

Applications Validated by Active Motif: ChIP-Seq: 6 ul per ChIP
Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen This BRD2 antibody was raised against a recombinant protein comprising amino acids 58-232 of human BRD2 (NP_005095 ).
Full Name BRD2 antibody (pAb)
Synonyms Bromodomain, BRD3, BRD4, BRD, chromodomain, Tudor domain, chromatin, histone, polyclonal antibody, chip, chip-seq, epigenetics
Molecular weight 110 kDa
GenBank accession numberNP_005095
RRIDAB_2793770
Purification Method Affinity Purified
Buffer Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

BRD2 (Bromodomain-containing protein 2) belongs to the BET subclass of proteins, which are characterized by two N-terminal bromodomains and one ET (Extra Terminal) domain. BRDs associate with chromatin through their bromodomains that recognize acetylated histone lysine residues. Bromodomains function as ‘readers’ of these epigenetic histone marks and regulate chromatin structure and gene expression by linking associated proteins to the acetylated nucleosomal targets. The ET domain functions as a protein binding motif and exerts atypical serine-kinase activity. The BET family consists of at least four members in mouse and human, BRD2 (also referred to as FSRG1, RING3), BRD3 (FSRG2, ORFX), BRD4 (FSRG4, MCAP/HUNK1), and BRDT (FSRG3, BRD6). BRD proteins are related to the female Sterile Homeotic protein in Drosophila, a gene required maternally for proper expression of other homeotic genes, such as Ubx, which is involved in pattern formation. BRD2 causes elevated protein kinase activity in leukemias. Transgenic mice overexpressing BRD2 in the lymphoid system develop diffuse large-cell lymphoma. BRD2 has been shown to interact with E2F1 and with histone H4 acetylated at Lys12 via its two bromodomains. BRD2 may play a role in spermatogenesis or folliculogenesis. Genetic evidence links the BRD2 gene to both juvenile myoclonic epilepsy and photoparoxysomal responses.