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BAP1 antibody (pAb)

Host / Isotype

Rabbit / IgG

Reactivity

Mouse

Applications

WB

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Cat No : 61501,61502 61501

Synonyms

BAP1, BRCA1 Associated Protein-1 (Ubiquitin Carboxy-Terminal Hydrolase), Ubiquitin Carboxyl-Terminal, Hydrolase BAP1, Cerebral Protein 6, HUCEP-13, UCHL2, BRCA1-Associated Protein 1, Cerebral Protein-13, deubiquitination, hcfc1, brca1, bard1, h2a, lysine 119, polyclonal, pab, antibody, western blotting, wb, sample

Validation Data Gallery

  • BAP1 antibody (pAb) tested by Western blot. Nuclear extract of P19 cells (25 ug) probed with the BAP1 antibody (1:1,000 dilution).

    BAP1 antibody (pAb) tested by Western blot. Nuclear extract of P19 cells (25 ug) probed with the BAP1 antibody (1:1,000 dilution).

Product Information

Tested Applications WB

Applications Validated by Active Motif: WB*: 1:500 - 1:2,000 dilution *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot. The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot.
Tested Reactivity Mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen This antibody was raised against a peptide within the C-terminal region of human BAP1.
Full Name BAP1 antibody (pAb)
Synonyms BAP1, BRCA1 Associated Protein-1 (Ubiquitin Carboxy-Terminal Hydrolase), Ubiquitin Carboxyl-Terminal, Hydrolase BAP1, Cerebral Protein 6, HUCEP-13, UCHL2, BRCA1-Associated Protein 1, Cerebral Protein-13, deubiquitination, hcfc1, brca1, bard1, h2a, lysine 119, polyclonal, pab, antibody, western blotting, wb, sample
Molecular weight 100 kDa
GenBank accession numberNP_004647
RRIDAB_2793661
Purification Method Affinity Purified
Buffer Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

BAP1 (BRCA1 Associated Protein-1 (Ubiquitin Carboxy-Terminal Hydrolase)) is a deubiquitinating enzyme that plays a key role in chromatin by mediating deubiquitination of histone H2A and HCFC1. Catalytic component of the PR-DUB complex, a complex that specifically mediates deubiquitination of histone H2A monoubiquitinated at 'Lys-119' (H2AK119ub1). Does not deubiquitinate monoubiquitinated histone H2B. Acts as a regulator of cell growth by mediating deubiquitination of HCFC1 N-terminal and C-terminal chains, with some specificity toward 'Lys-48'-linked polyubiquitin chains compared to 'Lys-63'-linked polyubiquitin chains. Deubiquitination of HCFC1 does not lead to increase stability of HCFC1. Interferes with the BRCA1 and BARD1 heterodimer activity by inhibiting their ability to mediate ubiquitination and autoubiquitination. It however does not mediate deubiquitination of BRCA1 and BARD1. Acts as a tumor suppressor.