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Aurora B antibody (pAb)

Host / Isotype

Rabbit / Serum

Reactivity

Human, Mouse, Wide Range Predicted

Applications

ICC, IF, WB

Activ Motif logo

Cat No : 39261,39262 39261

Synonyms

Aurora B, AuroraB, Aurora-B, mitosis, pAb, polyclonal, Aurora Kinase B, AurB, ARK2, antibody, antibodies, sample, centromere

Validation Data Gallery

  • Aurora B pAb tested by immunofluorescence. Top left: HeLa cells stained with Aurora B polyclonal antibody at a 1:200 dilution. Top right: Same cells stained with alpha Tubulin mAb. Bottom left: Same cells stained with DAPI. Bottom right: Merge of all 3 images.

    Aurora B pAb tested by immunofluorescence. Top left: HeLa cells stained with Aurora B polyclonal antibody at a 1:200 dilution. Top right: Same cells stained with alpha Tubulin mAb. Bottom left: Same cells stained with DAPI. Bottom right: Merge of all 3 images.

  • Aurora B pAb tested by Western blot Nuclear extract of U2OS cells (20 ug per lane) probed with Aurora B polyclonal antibody (1:500 dilution).

    Aurora B pAb tested by Western blot Nuclear extract of U2OS cells (20 ug per lane) probed with Aurora B polyclonal antibody (1:500 dilution).

Product Information

Tested Applications ICC, IF, WB

Applications Validated by Active Motif: ICC/IF: 1:200 - 1:1,000 dilution WB: 1:500 - 1:5,000 dilution
Tested Reactivity Human, Mouse, Wide Range Predicted
Host / Isotype Rabbit / Serum
Class Polyclonal
Type Antibody
Immunogen This Aurora B antibody was raised against a peptide including the C-terminus of human Aurora B.
Full Name Aurora B antibody (pAb)
Synonyms Aurora B, AuroraB, Aurora-B, mitosis, pAb, polyclonal, Aurora Kinase B, AurB, ARK2, antibody, antibodies, sample, centromere
Molecular weight 49 kDa
GenBank accession numberNP_004208
RRIDAB_2793209
Purification Method None
Buffer Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

Aurora B (Aurora Kinase B, AurB, ARK2) is a Ser/Thr kinase that is part of the chromosomal passenger complex that associates with chromosomes and is an important regulator of mitosis and meiosis. Aurora B becomes concentrated at the centromere during the onset of mitosis, but relocates to the spindle midzone during anaphase. Aurora B phosphorylates a number of substrates, including INCENP, CENP-A, and Histone H3. Misregulation of Aurora B activity can lead to chromosome instability and is implicated in a number of cancers.