Ago2 antibody (mAb) (Clone 1B1-E2H5)
Host / Isotype
Mouse / IgG2a
Reactivity
Human
Applications
ICC, IF, IP, WB
CloneNo.
1B1-E2H5
Cat No : 39853,39854 39853
Synonyms
Validation Data Gallery
Product Information
| Tested Applications | ICC, IF, IP, WB |
| Tested Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | This Ago2 antibody was raised against a recombinant protein corresponding to amino acids 1-148 of human Ago2. |
| Full Name | Ago2 antibody (mAb) (Clone 1B1-E2H5) |
| Synonyms | ago, argonaute, dicer, rnase, rnai, silencing, sirna, mirna, pri-mirna, risc, rna interference, ago2, argonaute 2, ago 2, argonaute 2, PTSG, sample |
| Molecular weight | 100 kDa |
| GenBank accession number | NP_036286 |
| RRID | AB_2793369 |
| Purification Method | Protein G Chromatography |
| Buffer | Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
| Storage | Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage. |
Background Information
Argonaute 2 (Ago2, aka EIF2C2) is a member of the Argonaute family of proteins involved in RNAi mediated gene silencing through siRNA and miRNA effectors. The Argonaute proteins are part of RISC, the RNAi Induced Silencing Complex. Binding of RISC can inhibit mRNA translation and results in the degradation of the mRNA through the action of Ago2-mediated target cleavage.