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Acf1 antibody (pAb)

Host / Isotype

Rabbit / IgG

Reactivity

Drosophila

Applications

ICC, IF, WB

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Cat No : 39721,39722 39721

Synonyms

Acf1, ATP-utilizing chromatin assembly and remodeling factor, ATP-dependent, nucleosome arrays, CHRAC (chromatin accessibility complex), repressive heterochromatin, sample

Validation Data Gallery

  • Acf1 pAb tested by immunofluorescence. Drosophila polytene chromosomes stained with Acf1 pAb (1:1,000 dilution).

    Acf1 pAb tested by immunofluorescence. Drosophila polytene chromosomes stained with Acf1 pAb (1:1,000 dilution).

  • Acf1 pAb tested by Western blot. Drosophila Schneider L2 cells nuclear extract (10 ug) probed with Acf1 pAb (1:500 dilution).

    Acf1 pAb tested by Western blot. Drosophila Schneider L2 cells nuclear extract (10 ug) probed with Acf1 pAb (1:500 dilution).

Product Information

Tested Applications ICC, IF, WB

Applications Validated by Active Motif: ICC/IF: 1:500 - 1:1,000 dilution WB: 1:500 - 1:1,000 dilution The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required.
Tested Reactivity Drosophila
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen This Acf1 antibody was raised against recombinant C-terminal fragment of Acf1 from Drosophila melanogaster.
Full Name Acf1 antibody (pAb)
Synonyms Acf1, ATP-utilizing chromatin assembly and remodeling factor, ATP-dependent, nucleosome arrays, CHRAC (chromatin accessibility complex), repressive heterochromatin, sample
Molecular weight 185 kDa
GenBank accession numberNP_536734
RRIDAB_2793321
Purification Method Affinity Purified
Buffer PBS containing 0.1% sodium azide, 0.2 mg/ml BSA and 35% glycerol. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

Drosophila Acf1 is a component of ACF, the ATP-utilizing chromatin assembly and remodeling factor protein complex. ACF catalyzes the ATP-dependent assembly of periodic nucleosome arrays in vitro. The catalytic subunit of ACF is the ISWI ATP-dependant chromatin remodeling protein. ACF is related to CHRAC (Chromatin Accessibility Complex), which also contains Acf1 and ISWI. ACF is involved in the establishment and possibly maintenance of silenced heterochromatic domains in the genome, both at pericentric heterochromatin and at genes repressed by the Polycomb complex.