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AbFlex Ubiquitin antibody (rAb)

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Wide Range Predicted

Applications

WB

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Cat No : 91257,91258 91257

Synonyms

ubiquitin, ubiquitylation, signaling, proteasome, ubiquitination,

Validation Data Gallery

  • AbFlex Ubiquitin antibody (rAb) tested by Western blot. 20 ug of HeLa cell nuclear acid-extract was run on SDS-PAGE and probed with AbFlex Ubiquitin antibody at 0.5 ug/ml. Antibodies to Ubiquitin will detect proteins of various sizes that are modified by the addition of Ubiquitin.

    AbFlex Ubiquitin antibody (rAb) tested by Western blot. 20 ug of HeLa cell nuclear acid-extract was run on SDS-PAGE and probed with AbFlex Ubiquitin antibody at 0.5 ug/ml. Antibodies to Ubiquitin will detect proteins of various sizes that are modified by the addition of Ubiquitin.

  • AbFlex Ubiquitin antibody specifity Dot blot analysis was used to confirm the specificity of Ubiquitin antibody. 50 ng, 10 ng, and 2 ng of either recombinant Ubiquitin (Lane 1) or BSA (Lane 2) were spotted onto nylon membrane and probed with AbFlex Ubiquitin recombinant antibody at 2 ug/ml.

    AbFlex Ubiquitin antibody specifity Dot blot analysis was used to confirm the specificity of Ubiquitin antibody. 50 ng, 10 ng, and 2 ng of either recombinant Ubiquitin (Lane 1) or BSA (Lane 2) were spotted onto nylon membrane and probed with AbFlex Ubiquitin recombinant antibody at 2 ug/ml.

Product Information

Tested Applications WB

Applications Validated by Active Motif: WB: 0.5 - 2 ug/ml DB: 2 ug/ml AbFlex recombinant antibodies are genetically derived from DNA sequences of parental hybridoma clones. For details on the parental clone, see Catalog No. 39741.
Tested Reactivity Human, Wide Range Predicted
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Immunogen This antibody was raised against bovine ubiquitin.
Full Name AbFlex Ubiquitin antibody (rAb)
Synonyms ubiquitin, ubiquitylation, signaling, proteasome, ubiquitination,
Molecular weight Not Applicable
GenBank accession numberN/A
RRIDAB_2793822
Purification Method Protein A Chromatography
Buffer Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035% sodium azide, 30% glycerol. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis Tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex Ubiquitin antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. Ubiquitin is an important signaling protein that is catalytically added to proteins on lysine residues to modify their function. Addition of multiple ubiquitin proteins in a string (poly-ubiquitylation) to proteins signals their destruction by the proteasome. Mono-ubiquitylation of proteins has a much different effect, more in keeping with post-translational modification of proteins by phosphorylation or acetylation. Mono-ubiquitylation is part of a number of signal transduction pathways, including those involving histone proteins and transcriptional regulation.