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AbFlex KDM5A antibody (rAb)

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

ChIP-Seq, WB

Activ Motif logo

Cat No : 91211,91212 91211

Synonyms

JARID1A / KDM5A, JARID1A, KDM5A, lysine (K)-specific demethylase 5A, Lysine demethylase 5A, jumonji, AT rich interactive domain 1A, Jumonji/ARID, ARID, lysine-specific demethylase 5A, RBBP2, histone lysine demethylase, lysine-specific demethylase 5A, retinoblastoma binding protein 2, RBBP-2, 31431, hdm, kdm, lysine demethylase, histone demethylase

Validation Data Gallery

  • AbFlex KDM5A antibody (rAb) tested by ChIP-Seq Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit (Cat. No. 53040) with 25 ug of chromatin from human H727 lung cell chromatin 4 ug of AbFlex KDM5A antibody. ChIP DNA was sequenced on the Illumina NextSeq and 13 million sequence tags were mapped to identify KDM5A binding sites on chromosome 8.

    AbFlex KDM5A antibody (rAb) tested by ChIP-Seq Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit (Cat. No. 53040) with 25 ug of chromatin from human H727 lung cell chromatin 4 ug of AbFlex KDM5A antibody. ChIP DNA was sequenced on the Illumina NextSeq and 13 million sequence tags were mapped to identify KDM5A binding sites on chromosome 8.

  • AbFlex KDM5A antibody (rAb) tested by Western blot. 40 ug of HeLa cell nuclear extract was run on SDS-PAGE and probed with AbFlex KDM5A antibody at 2 ug/ml. MW: 196 kDa

    AbFlex KDM5A antibody (rAb) tested by Western blot. 40 ug of HeLa cell nuclear extract was run on SDS-PAGE and probed with AbFlex KDM5A antibody at 2 ug/ml. MW: 196 kDa

  • Specificity of AbFlex KDM5A demonstrated by Western blot. 500 ng of various FLAG-tagged human full-length recombinant proteins was loaded per lane and probed with KDM5A antibody at 1ug/ml (top panel) or FLAG antibody (bottom panel). KDM5A antibody only detects KDM5A protein; the FLAG antibody shows the presence of all proteins on the blot. Lane 1: KDM3A (Cat. No. 31456). Lane 2: KDM3B (Cat. No. 31429). Lane 3: KDM4A (Cat. No. 31457). Lane 4: KDM4B Cat. No. 31501). Lane 5: KDM4C (Cat. No. 31458). Lane 6: KDM4D (Cat. No. 31459). Lane 7: KDM5A (Cat. No. 31431). Lane 8: KDM5B (Cat. No. 31432). Lane 9: KDM5C (Cat. No. 31433).

    Specificity of AbFlex KDM5A demonstrated by Western blot. 500 ng of various FLAG-tagged human full-length recombinant proteins was loaded per lane and probed with KDM5A antibody at 1ug/ml (top panel) or FLAG antibody (bottom panel). KDM5A antibody only detects KDM5A protein; the FLAG antibody shows the presence of all proteins on the blot. Lane 1: KDM3A (Cat. No. 31456). Lane 2: KDM3B (Cat. No. 31429). Lane 3: KDM4A (Cat. No. 31457). Lane 4: KDM4B Cat. No. 31501). Lane 5: KDM4C (Cat. No. 31458). Lane 6: KDM4D (Cat. No. 31459). Lane 7: KDM5A (Cat. No. 31431). Lane 8: KDM5B (Cat. No. 31432). Lane 9: KDM5C (Cat. No. 31433).

Product Information

Tested Applications ChIP-Seq, WB

Applications Validated by Active Motif: ChIP-Seq: 4 ug per ChIP WB*: 0.5 - 2 ug/mL For optimal results, we recommend the addition of 0.1% Tween 20 to all blocking solutions to reduce background. Individual optimization may be required. *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.
Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Immunogen This antibody was raised against full length, recombinant human KDM5A.
Full Name AbFlex KDM5A antibody (rAb)
Synonyms JARID1A / KDM5A, JARID1A, KDM5A, lysine (K)-specific demethylase 5A, Lysine demethylase 5A, jumonji, AT rich interactive domain 1A, Jumonji/ARID, ARID, lysine-specific demethylase 5A, RBBP2, histone lysine demethylase, lysine-specific demethylase 5A, retinoblastoma binding protein 2, RBBP-2, 31431, hdm, kdm, lysine demethylase, histone demethylase
Molecular weight 196 kDa
GenBank accession numberNP_001036068
RRIDAB_2793805
Purification Method Protein A Chromatography
Buffer Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035% sodium azide, 30% glycerol. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis Tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. KDM5A Lysine (K)-specific demethylase 5A (KDM5A), also known as Jumonji, AT rich interactive domain 1A (JARID1A), is a histone demethylase that specifically demethylates lysine 4 of histone H3 (H3K4), thereby playing a central role in defining the histone code. KDM5A demethylates trimethylated and dimethylated, but not monomethylated H3K4. It does not demethylate histone H3K9, H3K27, H3K36, H3K79 or H4K20. KDM5A may function to stimulate transcription mediated by nuclear receptors and may be involved in transcriptional regulation of HOX proteins during cell differentiation. Also, KDM5A may be involved in the transcriptional repression of cytokines such as CXCL12. It has been shown to bind directly with the Retinoblastoma (pRb) protein which regulates cell proliferation. KDM5A also interacts with Rhombotin-2 which functions distinctly in erythropoiesis and in T-cell leukemogenesis.