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AbFlex JMJD2B / KDM4B antibody (rAb)

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

IP, WB

Activ Motif logo

Cat No : 91305,91306 91305

Synonyms

JMJD, JMJD2B, KDM, AbFlex, Recombinant antibody, antibody

Validation Data Gallery

  • AbFlex JMJD2B / KDM4B antibody (rAb) tested by Western blot. 20 ug of K562 nuclear extract was run on SDS-PAGE and probed with AbFlex JMJD2B / KDM4B antibody at 2 ug/ml.

    AbFlex JMJD2B / KDM4B antibody (rAb) tested by Western blot. 20 ug of K562 nuclear extract was run on SDS-PAGE and probed with AbFlex JMJD2B / KDM4B antibody at 2 ug/ml.

  • AbFlex JMJD2B / KDM4B antibody (rAb) tested antibody tested by immunoprecipitation. 4 ug of AbFlex JMJD2B / KDM4B antibody (Lane 1) or an unrelated mouse IgG (Lane 2) was used to immunoprecipitate JMJD2B / KDM4B from 500 ug of K562 nuclear extract. The immunoprecipitated protein was detected by Western blotting using a JMJD2B / KDM4B antibody, Cat. No. 61211 at a dilution of 1:500.

    AbFlex JMJD2B / KDM4B antibody (rAb) tested antibody tested by immunoprecipitation. 4 ug of AbFlex JMJD2B / KDM4B antibody (Lane 1) or an unrelated mouse IgG (Lane 2) was used to immunoprecipitate JMJD2B / KDM4B from 500 ug of K562 nuclear extract. The immunoprecipitated protein was detected by Western blotting using a JMJD2B / KDM4B antibody, Cat. No. 61211 at a dilution of 1:500.

Product Information

Tested Applications IP, WB

Applications Validated by Active Motif: IP: 4 ug per IP WB*: 0.05 – 2 ug/ml *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot.
Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Immunogen This antibody was raised against full-length recombinant human lysine-specific demethylase 4B (KDM4B) protein.
Full Name AbFlex JMJD2B / KDM4B antibody (rAb)
Synonyms JMJD, JMJD2B, KDM, AbFlex, Recombinant antibody, antibody
Molecular weight 130 kDa
GenBank accession numberNP_055830
Purification Method Protein A Chromatography
Buffer 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035% sodium azide, and 30% glycerol. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis Tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex JMJD2B / KDM4B antibody was expressed in 293 cells and contains mouse immunoglobulin heavy and light chains. JMJD2B / KDM4B (JmjC domain-containing histone demethylation protein 3B, Jumonji domain-containing protein 2B, JHDM3B) is a histone demethylase that has been shown to demethylate histone H3 trimethylated at lysine 9. JMJD2B / KDM4B is physically associated with the MLL2 methyltransferase complex where it serves as a co-factor for ERa-mediated transcriptional activation.