AbFlex JMJD2A antibody (rAb)

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

ELISA, WB

Cat No : 91143,91144

Synonyms

JMJD2A, KDM4A, JHDM3A, Jmj, histone demethylase, Jumonji, JmjC, JmjN, H3K9me2, H3K9me3, H3K36me2, H3K36me3, PHD finger, Tudor domain, stem cells, antibody, clone N154/32, sample, recombinant, rAb, rec Ab, AbFlex

---

Validation Data Gallery

• 

  AbFlex JMJD2A antibody (rAb) tested by Western blot. HeLa cell nuclear extract (20 ug) probed with AbFlex JMJD2A antibody (rAb) at a dilution of 2 ug/ml.

• 

  AbFlex JMJD2A antibody (rAb) tested by Luminex bead-based specificity analysis. Luminex bead-based specificity analysis was used to confirm the specificity of AbFlexTM JMJD2A antibody (rAb) antibody for JMJD2A. Various proteins were conjugated to MagPlex Luminex beads and incubated with various amounts of AbFlexTM JMJD2A antibody (rAb). Protein-bound antibody was detected with anti-mouse IgG-Phycoerythrin and read in a Luminex instrument. Luminex is a registered trademark of Luminex Corporation.

---

Product Information

• Tested Applications: ELISA, WB

  Applications Validated by Active Motif: WB*: 1 - 2 ug/ml dilution Bead-based ELISA: 40 - 600 ng/ml The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required. *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot. AbFlex recombinant antibodies are genetically derived from DNA sequences of parental hybridoma clones. For details on the parental clone, see Catalog No. 39815.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2a
• Class: Recombinant
• Type: Antibody
• Immunogen: This JMJD2A antibody was raised against a recombinant protein corresponding to amino acids 586-674 of human JMJD2A.
• Full Name: AbFlex JMJD2A antibody (rAb)
• Synonyms: JMJD2A, KDM4A, JHDM3A, Jmj, histone demethylase, Jumonji, JmjC, JmjN, H3K9me2, H3K9me3, H3K36me2, H3K36me3, PHD finger, Tudor domain, stem cells, antibody, clone N154/32, sample, recombinant, rAb, rec Ab, AbFlex
• Molecular weight: 130 kDa
• GenBank accession number: NP_055478
• RRID: AB_2793788
• Purification Method: Protein A Chromatography
• Buffer: Purified IgG in 200 mM Hepes pH 7.5, 100 mm NaCl, 50 mM NaOAc, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
• Storage: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis Tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex JMJD2A antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. JMJD2A (KDM4A, JHDM3A) is a member of the Jmj-containing (Jumonji) class of histone demethylase proteins that are involved in the regulation of genome function through the removal of methyl groups from histones. JMJD2A specifically demethylates histone H3 dimethyl lysine 9 (H3K9me2), trimethyl lysine 9, (H3K9me3), dimethyl lysine 36 (H3K36me2) and trimethyl lysine 36 (H3K36me3). It also participates in transcriptional repression of ASCL2 and E2F-responsive promoters via the recruitment of histone deacetylases and NCOR1. JMJD2A contains JmjC and JmjN domains, two PHD fingers and two tudor domains.