AbFlex ING5 antibody (rAb)
Host / Isotype
Mouse / IgG2a
Reactivity
Human
Applications
WB
Cat No : 91329,91330 91329
Synonyms
Validation Data Gallery
Product Information
| Tested Applications |
WB
Applications Validated by Active Motif: WB: 0.5 - 2 ug/ml AbFlex recombinant antibodies are genetically derived from DNA sequences of parental hybridoma clones. For details on the parental clone, see Catalog No. 65685. |
| Tested Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | This antibody was raised against full length human recombinant ING5 (Inhibitor of growth protein 5) protein. |
| Full Name | AbFlex ING5 antibody (rAb) |
| Synonyms | 7A11, AbFlex, recombinant antibody, ING, |
| Molecular weight | 30 kDa |
| GenBank accession number | NP_001317091 |
| Purification Method | Protein A Chromatography |
| Buffer | 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035% sodium azide, and 30% glycerol. Sodium azide is highly toxic. |
| Storage | Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage. |
Background Information
AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis Tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex ING5 antibody was expressed in CHO cells, and contains mouse IgG2a heavy and light chains. ING5 (Inhibitor of Growth 5) possesses different structural and functional elements, including a PHD (plant homeodomain), an NCR (novel conserved region), a LZL (leucine zipper like) finger, and two NLS (nuclear localization sequence) motifs. ING proteins bind to methylated core histones through their PHD motif, notably ING5 reads histone H3 when di- or trimethylated at lysine 4 (H3K4me2/3). ING5 is a subunit of two distinct histone acetyltransferase (HAT) complexes with HBO1 or MOZ/MORF as the two catalytic subunits. These complexes contain two different alternative scaffolding proteins, i.e. a BRPF protein or a JADE protein, that determine their specificity towards different core histones. ING complexes appear to participate in transcription, for example by interacting with transcriptional regulators such as the tumor suppressor p53 and through the ability of ING5 to recognize H3K4me3, a mark associated with open chromatin and transcribed genes.