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AbFlex Histone H3K79me2 antibody   (rAb)

Host / Isotype

Rabbit / IgG2a

Reactivity

Human, Wide Range Predicted

Applications

ChIP, DB, WB

Activ Motif logo

Cat No : 91399,91400 91399

Synonyms

H3 trimethyl-lys79, Histone H3 dimethyl-lys79, dimethylated lysine 79, trimethylated lysine 79 of histone h3, K79, dmk79, tmk79, histone, histone h3, chromatin, histone methylation, nucleosome, western blotting, wb, polyclonal, pab, antibody, sample

Validation Data Gallery

  • AbFlex Histone H3K79me2 antibody (rAb) tested by ChIP-Seq Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit (Cat. No. 53040) with 30 ug of HeLa cell chromatin and 4 ug of antibody. ChIP DNA was sequenced on the Illumina NextSeq and 10 million sequence tags were mapped to identify Histone H3K79me2 binding sites on chromosome 6.

    AbFlex Histone H3K79me2 antibody (rAb) tested by ChIP-Seq Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit (Cat. No. 53040) with 30 ug of HeLa cell chromatin and 4 ug of antibody. ChIP DNA was sequenced on the Illumina NextSeq and 10 million sequence tags were mapped to identify Histone H3K79me2 binding sites on chromosome 6.

  • AbFlex Histone H3K79me2 antibody (rAb) tested by Western blot. 20 ug of HeLa cell nuclear extract* was run on SDS-PAGE and probed with AbFlex Histone H3K79me2 antibody at 0.5 ug/ml.

    AbFlex Histone H3K79me2 antibody (rAb) tested by Western blot. 20 ug of HeLa cell nuclear extract* was run on SDS-PAGE and probed with AbFlex Histone H3K79me2 antibody at 0.5 ug/ml.

  • AbFlex Histone H3K79me2 antibody specificity is shown by Dot Blot. Recombinant proteins were spotted onto Nitrocellulose as follows: Lane 1 - Histone H3 (C110A), Cat. No. 31207; Lane 2 - Histone H3K4me2 (EPL), Cat. No. 31277; Lane 3 - Histone H3K9me2 (EPL), Cat. No. 31280; Lane 4 - Histone H3K14me2 (MLA), Cat. No. 31257; Lane 5 - Histone H3K18me2 (MLA), Cat. No. 31260; Lane 6 - Histone H3K23me2 (MLA), Cat. No. 31263; Lane 7- Histone H3K27me2 (MLA), Cat. No. 31215; Lane 8 - Histone H3K36me2 (MLA), Cat. No. 31218; Lane 9 - Histone H3K79me1 (MLA), Cat. No. 31623; Lane 10 - Histone H3K79me2 (MLA), Cat. No. 31599; Lane 11 - Histone H3K79me3 (MLA), Cat. No. 31581; The blot was probed with AbFlex Histone H3K79me2 antibody at 1 ug/ml.

    AbFlex Histone H3K79me2 antibody specificity is shown by Dot Blot. Recombinant proteins were spotted onto Nitrocellulose as follows: Lane 1 - Histone H3 (C110A), Cat. No. 31207; Lane 2 - Histone H3K4me2 (EPL), Cat. No. 31277; Lane 3 - Histone H3K9me2 (EPL), Cat. No. 31280; Lane 4 - Histone H3K14me2 (MLA), Cat. No. 31257; Lane 5 - Histone H3K18me2 (MLA), Cat. No. 31260; Lane 6 - Histone H3K23me2 (MLA), Cat. No. 31263; Lane 7- Histone H3K27me2 (MLA), Cat. No. 31215; Lane 8 - Histone H3K36me2 (MLA), Cat. No. 31218; Lane 9 - Histone H3K79me1 (MLA), Cat. No. 31623; Lane 10 - Histone H3K79me2 (MLA), Cat. No. 31599; Lane 11 - Histone H3K79me3 (MLA), Cat. No. 31581; The blot was probed with AbFlex Histone H3K79me2 antibody at 1 ug/ml.

Product Information

Tested Applications ChIP, DB, WB

Applications Validated by Active Motif: ChIP-Seq: 4 ug per ChIP WB: 0.5 - 2 ug/ml dilution DB: 1 - 2 ug/ml dilution
Tested Reactivity Human, Wide Range Predicted
Host / Isotype Rabbit / IgG2a
Class Recombinant
Type Antibody
ModificationMethylated
Immunogen This Histone H3 dimethyl Lys79 antibody was raised against a peptide including dimethyl-lysine 79 of histone H3.
Full Name AbFlex Histone H3K79me2 antibody   (rAb)
Synonyms H3 trimethyl-lys79, Histone H3 dimethyl-lys79, dimethylated lysine 79, trimethylated lysine 79 of histone h3, K79, dmk79, tmk79, histone, histone h3, chromatin, histone methylation, nucleosome, western blotting, wb, polyclonal, pab, antibody, sample
Molecular weight 17 kDa
GenBank accession numberNP_003520
Purification Method Protein A Chromatography
Buffer 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035% sodium azide, and 30% glycerol.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis Tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex Histone H3K79me2 antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. Lysine 79 of histone H3 can be mono-, di- or trimethylated by Dot1 methylase; methylation at this residue acts as a marker of inactive chromatin regions that is critical for transcriptional silencing, and it is thought that silencing proteins such as Sir3 function by blocking Dot1 methylation.