AbFlex Histone H3K4me1 antibody (rAb)

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Wide Range Predicted

Applications

WB

Cat No : 91289,91290

Synonyms

histone H3, histone, H3, histone-H3, histoneH3, pAb, monoclonal, H3K4me1, Histone H3 monomethyl Lys4, Histone H3 monomethyl Lysine 4, Histone H3 monomethyl Lys 4, Histone H3 monomethyl K4, Histone H3 monomethyl K 4, Histone H3 mono-methyl Lys4, Histone H3 mono-methyl Lysine 4, Histone H3 mono-methyl Lys 4, Histone H3 mono-methyl K4, Histone H3 mono-methyl K 4, Histone H3 1Me Lys4, Histone H3 1Me Lysine 4, Histone H3 1Me Lys 4, Histone H3 1Me K4, Histone H3 1Me K 4, Histone H3 Me1 Lys4, Histone H3 Me1 Lysine 4, Histone H3 Me1 Lys 4, Histone H3 Me1 K4, Histone H3 Me1 K 4, antibody, antibodies, MABI, clone 0302, chip, chromatin immunoprecipitation, H3K4Me1, 61314

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Validation Data Gallery

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  AbFlex Histone H3K4me1 antibody (rAb) tested by Western blot. 20 ug of HeLa cell nuclear extract was run on SDS-PAGE and probed with AbFlex Histone H3K4me1 antibody at 0.5 ug/ml. Note: For optimal results, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for detection of Histone H3K4me1.

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  AbFlex Histone H3K4me1 antibody (rAb) specificity tested by peptide array analysis. Peptide array analysis was used to confirm the specificity of this antibody for its intended modification. Histone H3 trimethyl Lys27 antibody was applied at 0.2 ug/ml to Active Motif's MODified Histone Peptide Array (Catalog No. 13001). The arrays were scanned with ArrayAnalysis Software 7 and the results plotted. Recognition of the H3K4me1 peptides by the antibody is blocked by phosphorylation at Thr3.

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Product Information

• Tested Applications: WB

  Applications Validated by Active Motif: WB: 0.5 – 2 ug/ml Note: For optimal results, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.

• Tested Reactivity: Human, Wide Range Predicted
• Host / Isotype: Mouse / IgG2a
• Class: Recombinant
• Type: Antibody
• Modification: Methylated
• Immunogen: This antibody was raised against a peptide containing mono-methyl lysine 4 of Histone H3.
• Full Name: AbFlex Histone H3K4me1 antibody (rAb)
• Synonyms: histone H3, histone, H3, histone-H3, histoneH3, pAb, monoclonal, H3K4me1, Histone H3 monomethyl Lys4, Histone H3 monomethyl Lysine 4, Histone H3 monomethyl Lys 4, Histone H3 monomethyl K4, Histone H3 monomethyl K 4, Histone H3 mono-methyl Lys4, Histone H3 mono-methyl Lysine 4, Histone H3 mono-methyl Lys 4, Histone H3 mono-methyl K4, Histone H3 mono-methyl K 4, Histone H3 1Me Lys4, Histone H3 1Me Lysine 4, Histone H3 1Me Lys 4, Histone H3 1Me K4, Histone H3 1Me K 4, Histone H3 Me1 Lys4, Histone H3 Me1 Lysine 4, Histone H3 Me1 Lys 4, Histone H3 Me1 K4, Histone H3 Me1 K 4, antibody, antibodies, MABI, clone 0302, chip, chromatin immunoprecipitation, H3K4Me1, 61314
• Molecular weight: 17 kDa
• GenBank accession number: NP_003522
• RRID: AB_2793834
• Purification Method: Protein A Chromatography
• Buffer: 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035% sodium azide, and 30% glycerol. Sodium azide is highly toxic.
• Storage: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis Tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex Histone H3K4me1 antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.