AbFlex Histone H3K27me3 antibody (rAb)
Host / Isotype
Rabbit / IgG
Reactivity
Human, Wide Range Predicted
Applications
DB, WB
Cat No : 91167,91168 91167
Synonyms
Validation Data Gallery
Product Information
| Tested Applications |
DB, WB
Applications Validated by Active Motif: WB: 0.1 - 1 ug/ml Note: For optimal results, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot. |
| Tested Reactivity | Human, Wide Range Predicted |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Modification | Methylated |
| Immunogen | This Histone H3 trimethyl Lys27 antibody was raised against a peptide containing trimethyl Lys27 of human Histone H3. |
| Full Name | AbFlex Histone H3K27me3 antibody (rAb) |
| Synonyms | H3K27me3, histone, methylation, demethylation, recombinant antibody, AbFlex |
| Molecular weight | 17 kDa |
| GenBank accession number | NP_003522 |
| RRID | AB_2793791 |
| Purification Method | Protein A Chromatography |
| Buffer | 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035% sodium azide, and 30% glycerol. Sodium azide is highly toxic. |
| Storage | Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage. |
Background Information
AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis Tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex Histone H3K27me3 antibody was expressed in 293 cells, and contains rabbit immunoglobulin heavy and light chains. Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). The methylation of histones can occur on two different residues: arginine or lysine. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residue. Lysine 27 of histone H3 can be mono-, di- or trimethylated (Histone H3 monomethyl Lys27, Histone H3 dimethyl Lys27 or Histone H3 trimethyl Lys27) by different histone methyltransferases such as EZH2 or NSD3. Methylation of this residue is mainly associated with transcriptional repression.