AbFlex Histone H2A antibody (rAb)

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Wide Range Predicted

Applications

WB

Cat No : 91325,91326

Synonyms

histone H2A, H2, histone, histones, H2A, histone-H2A, histoneH2A, AbFlex

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Validation Data Gallery

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  AbFlex Histone H2A antibody tested by Western blot. 20 ug of HeLa nuclear extract* was run on SDS-PAGE and probed with antibody at 0.5 ug/ml.

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  AbFlex Histone H2A antibody specificity AbFlex Histone H2A antibody (rAb) tested by Luminex bead-based specificity analysis. Luminex bead-based specificity analysis was used to confirm the specificity of AbFlex Histone H2A antibody (rAb) for Histone H2A. Various Histone proteins were conjugated to MagPlex Luminex beads and incubated with various amounts of AbFlex Histone H2A antibody (rAb). Protein-bound antibody was detected with anti-mouse IgG-Phycoerythrin and read in a Luminex instrument. Luminex is a registered trademark of Luminex Corporation.

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Product Information

• Tested Applications: WB

  Applications Validated by Active Motif: WB*: 0.5 – 2 ug/ml *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.

• Tested Reactivity: Human, Wide Range Predicted
• Host / Isotype: Mouse / IgG2a
• Class: Recombinant
• Type: Antibody
• Immunogen: This antibody was raised against recombinant human Histone H2A.
• Full Name: AbFlex Histone H2A antibody (rAb)
• Synonyms: histone H2A, H2, histone, histones, H2A, histone-H2A, histoneH2A, AbFlex
• Molecular weight: 17 kDa
• GenBank accession number: NP_003503
• Purification Method: Protein A Chromatography
• Buffer: 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035% sodium azide, and 30% glycerol. Sodium azide is highly toxic.
• Storage: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis Tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex Histone H2A antibody was expressed in was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) CHO cells.