AbFlex HIF-1 alpha antibody (rAb)

Host / Isotype

Mouse / IgG2a

Reactivity

Bovine, Human, Mouse, Rat

Applications

ELISA

Cat No : 91139,91140

Synonyms

HIF-1 alpha, hypoxia inducible factor 1, alpha subunit, MOP1, PASD8, bHLHe78, PAS domain-containing protein 8, HIF1, antibody, sample, Immunohistochemistry, IHC, transcription, oxidative stress, recombinant, rAb, rec Ab, AbFlex

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Validation Data Gallery

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  AbFlex HIF-1 alpha antibody (rAb) tested by Luminex bead-based specificity analysis. Luminex bead-based specificity analysis was used to confirm the specificity of AbFlex HIF-1 alpha antibody (rAb) antibody for HIF-1 alpha. Various proteins were conjugated to MagPlex Luminex beads and incubated with various amounts of AbFlex HIF-1 alpha antibody (rAb). Protein-bound antibody was detected with anti-mouse IgG-Phycoerythrin and read in a Luminex instrument. Luminex is a registered trademark of Luminex Corporation.

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Product Information

• Tested Applications: ELISA

  Applications Validated by Active Motif: Bead-based ELISA: 10 - 76 ng/ml AbFlex recombinant antibodies are genetically derived from DNA sequences of parental hybridoma clones. For details on the parental clone, see Catalog No. 61275.

• Tested Reactivity: Bovine, Human, Mouse, Rat
• Host / Isotype: Mouse / IgG2a
• Class: Recombinant
• Type: Antibody
• Immunogen: This HIF-1 alpha antibody was raised against a partial protein containing amino acid residues 329-530 of human HIF-1 alpha.
• Full Name: AbFlex HIF-1 alpha antibody (rAb)
• Synonyms: HIF-1 alpha, hypoxia inducible factor 1, alpha subunit, MOP1, PASD8, bHLHe78, PAS domain-containing protein 8, HIF1, antibody, sample, Immunohistochemistry, IHC, transcription, oxidative stress, recombinant, rAb, rec Ab, AbFlex
• Molecular weight: 100 kDa
• GenBank accession number: NP_001521
• RRID: AB_2793787
• Purification Method: Ni-NTA
• Buffer: Purified IgG in 200 mM Hepes pH 7.5, 100 mm NaCl, 50 mM NaOAc, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
• Storage: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis Tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex HIF-1 alpha antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. HIF-1 (hypoxia inducible factor 1, alpha subunit, MOP1, PASD8, bHLHe78, PAS domain-containing protein 8) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF-1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT). This gene encodes the alpha subunit of HIF-1. Overexpression of a natural antisense transcript (aHIF) of this gene has been shown to be associated with nonpapillary renal carcinomas. Two alternative transcripts encoding different isoforms have been identified.