AbFlex DNMT1 antibody (rAb)
Host / Isotype
Mouse / IgG
Reactivity
Human
Applications
WB
Cat No : 91175,91176 91175
Synonyms
Validation Data Gallery
Product Information
| Tested Applications |
WB
Applications Validated by Active Motif: WB: 0.5 – 2 ug/ml AbFlex recombinant antibodies are genetically derived from DNA sequences of parental hybridoma clones. For details on the parental clone, see Catalog No. 61467. |
| Tested Reactivity | Human |
| Host / Isotype | Mouse / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | This antibody was raised against recombinant protein comprising full length human DNMT1 (DNA (cytosine-5)-methyltransferase 1). The antibody does not react with mouse Dnmt1. |
| Full Name | AbFlex DNMT1 antibody (rAb) |
| Synonyms | DNMT1, DNMT 1, DNA methyltransferase 1, DNA (Cytosine-5-)-Methyltransferase 1, DNA MTase HsaI, MCMT, CXXC9, HSN1E, DNMT, DNA (Cytosine-5)-Methyltransferase 1, AIM, M.HsaI, CXXC-Type Zinc Finger Protein 9, Dnmt1, DNA Methyltransferase, AbFlex recombinant antibodies |
| Molecular weight | 190 kDa |
| GenBank accession number | NP_001124295 |
| Purification Method | Protein A Chromatography |
| Buffer | Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035% sodium azide, 30% glycerol. Sodium azide is highly toxic. |
| Storage | Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage. |
Background Information
AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis Tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex DNMT1 antibody was expressed in CHO cells and contains mouse immunoglobulin heavy and light chains. DNMT1 (DNA (cytosine-5)-methyltransferase) proteins are involved in DNA methylation in which a methyl group is added to a cytosine residue on DNA, commonly at the C5 position of a CpG dinucleotide. Three families of DNMTs have been identified: DNMT1, DNMT2, and DNMT3 (comprised of DNMT3A and DNMT3B). DNMT1 is the most abundant DNMT in somatic cells and shows a preference for methylating hemi-methylated DNA. It is considered to be a maintenance DNA methyltransferase that is important in the maintenance of specific patterns of methylation throughout cellular divisions. Methylation of mammalian DNA has long been recognized to play a major role in a number of cellular functions such as embryonic development, genetic imprinting, X chromosome inactivation and the control of gene expression. DNA methylation is generally associated with transcriptional repression.