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AbFlex CTCF antibody (rAb)

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

ChIP-Seq, CUT&Tag, WB

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Cat No : 91285,91286 91285

Synonyms

CTCF, CCCTC-binding factor (zinc finger protein), 11-zinc finger protein, transcriptional repressor CTCF, CCCTC-binding factor, CTCFL paralog, 11 zinc finger transcriptional repressor, ChIP, chromatin immunoprecipitation, western blotting, pAb, polyclonal, antibody, transcription, chromatin, sample, chiphsabs

Validation Data Gallery

  • AbFlex CTCF recombinant antibody (rAb) tested by ChIP-Seq Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit (Cat. No. 53040) with 30 ug of MCF7 chromatin and 4 ug of CTCF antibody. ChIP DNA was sequenced on the Illumina NextSeq and 8.9 million sequence tags were mapped to identify CTCF binding sites on chromosome 9.

    AbFlex CTCF recombinant antibody (rAb) tested by ChIP-Seq Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit (Cat. No. 53040) with 30 ug of MCF7 chromatin and 4 ug of CTCF antibody. ChIP DNA was sequenced on the Illumina NextSeq and 8.9 million sequence tags were mapped to identify CTCF binding sites on chromosome 9.

  • AbFlex CTCF antibody (rAb) tested by Western blot. 20 ug of HeLa cell nuclear extract was run on SDS-PAGE and probed with AbFlex CTCF antibody at 0.2 ug/ml.

    AbFlex CTCF antibody (rAb) tested by Western blot. 20 ug of HeLa cell nuclear extract was run on SDS-PAGE and probed with AbFlex CTCF antibody at 0.2 ug/ml.

  • AbFlex CTCF recombinant antibody (rAb) tested by CUT&Tag CUT&Tag was performed using 250,000 K562 cells and sequenced using 38 base-pair, paired-end reads on the Illumina NextSeq 500/550. Data was collected from 21 million reads, and CTCF data is shown for Chromosome 1.

    AbFlex CTCF recombinant antibody (rAb) tested by CUT&Tag CUT&Tag was performed using 250,000 K562 cells and sequenced using 38 base-pair, paired-end reads on the Illumina NextSeq 500/550. Data was collected from 21 million reads, and CTCF data is shown for Chromosome 1.

Product Information

Tested Applications ChIP-Seq, CUT&Tag, WB

Applications Validated by Active Motif: ChIP-Seq: 4 ug per ChIP WB: 0.1-1 ug/ml CUT&Tag: 1 ul per 50 ul reaction Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts.
Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen This antibody was raised against a peptide within human CTCF.
Full Name AbFlex CTCF antibody (rAb)
Synonyms CTCF, CCCTC-binding factor (zinc finger protein), 11-zinc finger protein, transcriptional repressor CTCF, CCCTC-binding factor, CTCFL paralog, 11 zinc finger transcriptional repressor, ChIP, chromatin immunoprecipitation, western blotting, pAb, polyclonal, antibody, transcription, chromatin, sample, chiphsabs
Molecular weight 120 kDa
GenBank accession numberNP_006556
Purification Method Protein A Chromatography
Buffer Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035% sodium azide, 30% glycerol. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis Tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex CTCF antibody was expressed in CHO cells and contains rabbit immunoglobulin heavy and light chains. CTCF (CCCTC-binding factor, zinc finger protein) is a chromatin binding factor that binds to DNA sequence specific sites. It is involved in transcriptional regulation by binding to chromatin insulators and preventing interaction between promoter and nearby enhancers and silencers. Preferentially interacts with unmethylated DNA, preventing spreading of CpG methylation and maintaining methylation-free zones. Inversely, binding to target sites is prevented by CpG methylation. Plays a important role in chromatin remodeling. Can dimerize when it is bound to different DNA sequences, mediating long-range chromatin looping.