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AbFlex Ago 1/2/3 antibody (rAb)

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

IP

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Cat No : 91217,91218 91217

Synonyms

Ago1, Ago2, Ago3, argonaute, RNAi, siRNA, miRNA, RISC, RNAi Induced Silencing Complex,rna interference, rna processing

Validation Data Gallery

  • AbFlex Ago1/2/3 antibody (rAb) tested antibody tested by immunoprecipitation. Active Motif miRNA IP Kit (Cat. No. 25500) was used with 293 cells (10 million cells per IP). Following IP using AbFlex Ago1/2/3 antibody (5 ug per IP) or a negative control IgG, qRT-PCR was performed on samples using primers for IGF2. Fold enrichment over the negative control IgG shows successful specific IP of IGF2 miRNA associated with the RISC complex.

    AbFlex Ago1/2/3 antibody (rAb) tested antibody tested by immunoprecipitation. Active Motif miRNA IP Kit (Cat. No. 25500) was used with 293 cells (10 million cells per IP). Following IP using AbFlex Ago1/2/3 antibody (5 ug per IP) or a negative control IgG, qRT-PCR was performed on samples using primers for IGF2. Fold enrichment over the negative control IgG shows successful specific IP of IGF2 miRNA associated with the RISC complex.

Product Information

Tested Applications IP

Applications Validated by Active Motif: IP: 5 ug per sample AbFlex recombinant antibodies are genetically derived from DNA sequences of parental hybridoma clones. For details on the parental clone, see Catalog No. 39937.
Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Immunogen This Ago1/2/3 antibody was raised against a recombinant protein corresponding to amino acids 47-859 of human Ago2.
Full Name AbFlex Ago 1/2/3 antibody (rAb)
Synonyms Ago1, Ago2, Ago3, argonaute, RNAi, siRNA, miRNA, RISC, RNAi Induced Silencing Complex,rna interference, rna processing
Molecular weight
GenBank accession numberNP_036286
RRIDAB_2793808
Purification Method Protein A Chromatography
Buffer Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035% sodium azide, 30% glycerol. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis Tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex Ago 1/2/3 antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. The Argonaute family of proteins (Ago1, Ago2, Ago3 and Ago4) are involved in RNAi mediated gene silencing through siRNA and miRNA effectors. The Argonaute proteins are part of RISC, the RNAi Induced Silencing Complex. Binding of RISC can inhibit mRNA translation and results in the degradation of the mRNA through the action of Ago2-mediated target cleavage. Ago1 is non-essential for siRNA-directed target RNA cleavage but is needed for the production of mature miRNA that affects miRNA-directed RNA cleavage. Ago3 lacks RNase activity and does not appear to cleave target mRNA molecules. Argonaute family proteins contain a PAZ and a PIWI domain.