AbFlex 5-methylcytosine antibody (rAb)

Host / Isotype

Mouse / IgG2a

Reactivity

Not Species Specific

Applications

DB

Cat No : 91187,91188

Synonyms

5-methylcytidine, methyl cytidine, 5 MeCyd, 5-Me, 5 MeC, MeDIP, methylated DNA immunoprecipitation, DNA methylation, monoclonal, mouse, mAb, antibody, antibodies, methylcytosine, stem cell, stem cells

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Validation Data Gallery

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  Dot blot of AbFlex 5-methylcytosine antibody (rAb). DNA standards from Cat# 55008 (5, 1 and 0.2 ng as indicated) were spotted onto positively charged nylon membrane and blotted with AbFlex 5-methylcytosine recombinant antibody at a 1 ug/mL. Lane 1: Unmethylated DNA. Lane 2: DNA containing 5-methylcytosine. Lane 3: DNA containing 5-hydroxymethylcytosine.

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Product Information

• Tested Applications: DB

  Applications Validated by Active Motif: Dot Blot: 1 ug/ml

• Tested Reactivity: Not Species Specific
• Host / Isotype: Mouse / IgG2a
• Class: Recombinant
• Type: Antibody
• Modification: Methylated
• Immunogen: This antibody was raised against 5-methylcytosine conjugated to KLH.
• Full Name: AbFlex 5-methylcytosine antibody (rAb)
• Synonyms: 5-methylcytidine, methyl cytidine, 5 MeCyd, 5-Me, 5 MeC, MeDIP, methylated DNA immunoprecipitation, DNA methylation, monoclonal, mouse, mAb, antibody, antibodies, methylcytosine, stem cell, stem cells
• GenBank accession number: N/A
• RRID: AB_2793794
• Purification Method: Protein A Chromatography
• Buffer: Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035% sodium azide, 30% glycerol. Sodium azide is highly toxic.
• Storage: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis Tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex 5-methylcytosine (5-mC) antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. 5-Methylcytosine (5-Methylcytidine) is a modified base that is found in the DNA of plants and vertebrates. DNA methylation is an epigenetic event in which DNA methyltransferases (DNMTs) catalyze the reaction of a methyl group to the fifth carbon of cytosine in a CpG dinucleotide. This modification helps to control gene expression and is also involved in genomic imprinting, while aberrant DNA methylation is often associated with disease. This antibody is useful for discrimination between the modified base and its normal cytosine counterpart, allowing for gene promoter methylation analysis.