AEBP2 antibody (pAb)

Host / Isotype

Rabbit / IgG

Reactivity

Mouse

Applications

WB

Cat No : 61539,61540

Synonyms

AEBP2, AE Binding Protein 2, Adipocyte Enhancer-Binding Protein 2, AE-Binding Protein 2, AE(Adipocyte Enhancer)-Binding Protein 2, Zinc Finger Protein AEBP22, transcription, repressor, antibody, pab, polyclonal, western blotting, wb, samples

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Validation Data Gallery

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  AEBP2 antibody (pAb) tested by Western blot. AEBP2 detection by Western blot. The analysis was performed using 20 ug P19 nuclear cell extract and AEBP2 antibody at a 1:2,500 dilution. An unspecified band is detected around 160 kDa.

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Product Information

• Tested Applications: WB

  Applications Validated by Active Motif: WB*: 1:1,000 - 1:5,000 dilution The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required. *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.

• Tested Reactivity: Mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: This antibody was raised against a peptide within the C-terminal region of mouse AEBP2.
• Full Name: AEBP2 antibody (pAb)
• Synonyms: AEBP2, AE Binding Protein 2, Adipocyte Enhancer-Binding Protein 2, AE-Binding Protein 2, AE(Adipocyte Enhancer)-Binding Protein 2, Zinc Finger Protein AEBP22, transcription, repressor, antibody, pab, polyclonal, western blotting, wb, samples
• Molecular weight: 52 kDa
• GenBank accession number: NP_001005605
• RRID: AB_2614969
• Purification Method: Affinity Purified
• Buffer: Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
• Storage: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

AEBP2 (AE binding protein 2) is a DNA-binding transcriptional repressor and component of the PRC2 complex, which methylates 'Lys-27' residues of Histone H3.