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5-Methylcytosine (5-mC) antibody (mAb) (Clone 33D3)

Host / Isotype

Mouse / IgG

Reactivity

Human, Not Species Specific

Applications

DB, ELISA, FC, ICC, IF, IHC, MeDIP

CloneNo.

33D3

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Cat No : 39649 39649

Synonyms

5-methylcytosine, methyl cytidine, 5 MeCyd, 5-Me, 5 MeC, MeDIP, methylated DNA immunoprecipitation, DNA methylation, monoclonal, mouse, mAb, antibody, antibodies, methylcytidine, epigenetic, epigenetics, 5-mc, 5mc, Methylcytosine

Validation Data Gallery

  • 5-Methylcytosine antibody (mAb) (Clone 33D3) tested by Methyl-DNA Immunoprecipitation (MeDIP). Human DNA (500 ng) digested with Mse I was subjected to MeDIP and then analyzed by quantitative real-time PCR with primers specific for genes that are normally methylated (Xist, NYESO and SNRPN) or unmethylated (APC and p48). The MeDIP'd DNA (enriched DNA) was plotted.

    5-Methylcytosine antibody (mAb) (Clone 33D3) tested by Methyl-DNA Immunoprecipitation (MeDIP). Human DNA (500 ng) digested with Mse I was subjected to MeDIP and then analyzed by quantitative real-time PCR with primers specific for genes that are normally methylated (Xist, NYESO and SNRPN) or unmethylated (APC and p48). The MeDIP'd DNA (enriched DNA) was plotted.

  • 5-Methylcytosine (5-mc) antibody (pAb) tested by Immunohistochemistry Punctate nuclear staining pattern is detected in Formalin-fixed, paraffin-embedded tissue sections from human substantia nigra (midbrain). Top Panel: 5-mC antibody at 1:1000 dilution. Bottom Panel: No primary antibody (2nd step antibody alone)

    5-Methylcytosine (5-mc) antibody (pAb) tested by Immunohistochemistry Punctate nuclear staining pattern is detected in Formalin-fixed, paraffin-embedded tissue sections from human substantia nigra (midbrain). Top Panel: 5-mC antibody at 1:1000 dilution. Bottom Panel: No primary antibody (2nd step antibody alone)

Product Information

Tested Applications DB, ELISA, FC, ICC, IF, IHC, MeDIP

Validated Applications: MeDIP: 1 ug per IP IHC (FFPE): 1:1000 ELISA: 1:10,000 dilution Published Applications: MeDIP ICC/IF Flow Cytometry IHC DB
Tested Reactivity Human, Not Species Specific
Host / Isotype Mouse / IgG
Class Monoclonal
Type Antibody
ModificationMethylated
Immunogen Clone 33D3 recognizes the modified base 5-methylcytidine found in plant and vertebrate DNA.
Full Name 5-Methylcytosine (5-mC) antibody (mAb) (Clone 33D3)
Synonyms 5-methylcytosine, methyl cytidine, 5 MeCyd, 5-Me, 5 MeC, MeDIP, methylated DNA immunoprecipitation, DNA methylation, monoclonal, mouse, mAb, antibody, antibodies, methylcytidine, epigenetic, epigenetics, 5-mc, 5mc, Methylcytosine
Molecular weight
GenBank accession numberN/A
RRIDAB_2687950
Purification Method Protein A Chromatography
Buffer Purified IgG in 10 mM phosphate, 0.15 M NaCl and 0.01% thimerosal. Thimerosal is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

5-Methylcytosine (5-Methylcytidine) is a modified base that is found in the DNA of plants and vertebrates. DNA methylation is an epigenetic event in which DNA methyltransferases (DNMTs) catalyze the reaction of a methyl group to the fifth carbon of cytosine in a CpG dinucleotide. This modification helps to control gene expression and is also involved in genomic imprinting, while aberrant DNA methylation is often associated with disease. The 5-methylcytidine antibody (Clone 33D3) has been developed to discriminate between the modified base and its normal cytosine counterpart, allowing for gene promoter methylation analysis.