5-Carboxylcytosine (5-caC) antibody (pAb)

Host / Isotype

Rabbit / IgG

Reactivity

Not Species Specific

Applications

DB, ICC, IF

Cat No : 61229,61230

Synonyms

5-carboxylcytidine, 5-carboxylcytosine, 5-fC, 5fc, TET, dna methylation, carboxylcytosine, stem cell, stem cells, embryogenesis, 5-cac, 5cac, sample

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Validation Data Gallery

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  5-Carboxylcytosine antibody (pAb) tested by DNA dot blot Single-stranded DNA oligonucleotides (amount of oligo in nanograms listed on the left side of the blot) corresponding to the immunogen and related sequences were spotted onto nitrocellulose and probed with the antibody at 1:2,000. Lane 1: oligo containing 5-carboxylcytidine. Lane 2: oligo containing 5-formylcytidine. Lane 3: oligo containing 5-hydroxymethylcytidine. Lane 4: oligo containing 5-methylcytidine. Lane 5: oligo containing unmodified cytidine.

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Product Information

• Tested Applications: DB, ICC, IF

  Applications Validated by Active Motif: DB: 0.5 - 2 ug/ml dilution

• Tested Reactivity: Not Species Specific
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Modification: Methylated
• Immunogen: This 5-Carboxylcytosine antibody was raised against 5-carboxylcytidine conjugated to KLH and recognizes 5-carboxylcytosine.
• Full Name: 5-Carboxylcytosine (5-caC) antibody (pAb)
• Synonyms: 5-carboxylcytidine, 5-carboxylcytosine, 5-fC, 5fc, TET, dna methylation, carboxylcytosine, stem cell, stem cells, embryogenesis, 5-cac, 5cac, sample
• GenBank accession number: N/A
• RRID: AB_2793559
• Purification Method: Protein A Chromatography
• Buffer: Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
• Storage: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

DNA methylation is an epigenetic event in which DNA methyltransferases (DNMTs) catalyze the reaction of a methyl group to the fifth carbon of cytosine in a CpG dinucleotide. This modification helps to control gene expression and is also involved in genomic imprinting, while aberrant DNA methylation is often associated with disease. 5-methylcytosine is a modified base that is found in the DNA of plants and vertebrates. A second type of DNA methylation exists, 5-hydroxymethylcytosine (5-hydroxy methylcytosine, 5-hmC). This results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of cytosine oxygenases. Iterative activity of TET on 5-hydroxymethylcytosine results in the conversion of 5-hmC into 5-formylcytosine and 5-carboxylcytosine. An increase in levels of 5-formylcytosine and 5-carboxylcytosine are detected in the mouse male pronucleus following fertilization, which is gradually diluted by DNA replication.