3-Methylcytosine (3-mC) antibody (pAb)
Host / Isotype
Rabbit / IgG
Reactivity
Human, Mouse, Not Species Specific
Applications
DB, ICC, IF
Cat No : 61111,61112 61111
Synonyms
Validation Data Gallery
Product Information
Tested Applications |
DB, ICC, IF
Applications Validated by Active Motif: DB: 0.1 - 1 ug/ml dilution |
Tested Reactivity | Human, Mouse, Not Species Specific |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Modification | Methylated |
Immunogen | This 3-methylcytosine antibody was raised against 3-methylcytidine conjugated to KLH and recognizes 3-methylcytosine. |
Full Name | 3-Methylcytosine (3-mC) antibody (pAb) |
Synonyms | DNA methylation, S-adenosyl methionine, SAM, 3-methylcytosine, 3-meC, 3 methylcytosine, 3-methycytidine, 3 methylcytosine, 3-methylC, base excision repair, BER, dealkylation, AlkB, methylcytosine, sample |
Molecular weight | |
GenBank accession number | N/A |
RRID | AB_2793510 |
Purification Method | Protein A Chromatography |
Buffer | Purified rabbit IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. |
Storage | Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage. |
Background Information
Methylation of DNA can occur non-enzymatically at the nitrogen-three of the cytosine base through spontaneous exposure to endogenous S-adenosyl methionine (SAM). The resulting 3-methylcytosine (3-mC) is mutagenic and must be repaired, which occurs in humans through the base excision repair (BER) or dealkylation via human homologues of the E. coli AlkB protein. 3-methylcytosine is present in human cell lines and increased levels of 3-mC impair proliferation.