TIGIT Monoklonaler Antikörper

TIGIT Monoklonal Antikörper für FC

Wirt / Isotyp

Maus / IgG1, kappa

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

PE Fluorescent Dye

CloneNo.

1G9

Kat-Nr. : PE-65079

Synonyme

ENSMUSG00000071552, Tigit, Vstm3

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Galerie der Validierungsdaten

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  FC experiment of BALB/c mouse splenocytes using PE-65079

  1X10^6 Con A-stimulated (3 ug/ml, 2.5 days) BALB/c mouse splenocytes were surface stained with CoraLite® Plus 647-Conjugated Anti-Mouse CD3 (CL647-65077, Clone: 17A2) and 0.5 ug PE Anti-Mouse TIGIT (PE-65079, Clone: 1G9). Cells were not fixed.

• 

  FC experiment of BALB/c mouse splenocytes using PE-65079

  1X10^6 Con A-stimulated (3 ug/ml, 2.5 days) BALB/c mouse splenocytes were surface stained with CoraLite® Plus 647-Conjugated Anti-Mouse CD3 (CL647-65077, Clone: 17A2) and 0.5 ug PE-Mouse IgG1 isotype control. Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Maus-Splenozyten

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

PE-65079 bindet in FC TIGIT und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Maus / IgG1, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: k.A.
• Vollständiger Name: T cell immunoreceptor with Ig and ITIM domains
• GenBank-Zugangsnummer: NM_001146325
• Gene symbol: Tigit
• Gene ID (NCBI): 100043314
• Konjugation: PE Fluorescent Dye
• Excitation/Emission maxima wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: Phosphatbasierter Puffer mit 0,09% Natriumazid und 0,1% Gelatine, pH 7,2.
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TIGIT (T-cell immunoreceptor with Ig and ITIM domains), also known as VSIG9 or VSTM3, is an immune receptor expressed on T cells, including Treg and memory subsets, as well as on NK cells (PMID: 19011627). It contains an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT binds to poliovirus receptor (PVR, also called CD155) with high affinity, and also to PVRL2 (CD112) with lower affinity. The interaction of TIGIT with PVR on dendritic cells increases the secretion of IL-10 and decreases the secretion of proinflammatory cytokine and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells. TIGIT can inhibit NK cytotoxicity directly through its ITIM (PMID: 19815499).