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  • Featured Product
  • KD/KO Validated

Spartin, SPG20 Polyklonaler Antikörper

Spartin, SPG20 Polyklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF, FC (Intra)

Konjugation

CoraLite® Plus 647 Fluorescent Dye

Kat-Nr. : CL647-13791

Synonyme

KIAA0610, SPARTIN, Spartin, SPG20, Spastic paraplegia 20 protein, SPG20, TAHCCP1

Galerie der Validierungsdaten

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 647-conjugated Spartin, SPG20 Polyc (CL647-13791)

    IF Staining of HepG2 using CL647-13791

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite® Plus 647 Spartin, SPG20 antibody (CL647-13791) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite® Plus 647-conjugated Spartin, SPG20 Polyc (CL647-13791)

    FC experiment of HepG2 using CL647-13791

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 647 Anti-Human Spartin, SPG20 (CL647-13791) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Geprüfte Anwendungen

Erfolgreiche Detektion in IFHepG2-Zellen
Erfolgreiche Detektion in FCHepG2-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Produktinformation

CL647-13791 bindet in IF, FC (Intra) Spartin, SPG20 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Spartin, SPG20 fusion protein Ag4815
Vollständiger Name spastic paraplegia 20 (Troyer syndrome)
Berechnetes Molekulargewicht 75 kDa, 85 kDa
Beobachtetes Molekulargewicht 75 kDa, 84 kDa
GenBank-ZugangsnummerBC047083
Gene symbol SPG20
Gene ID (NCBI) 23111
Konjugation CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SPG20 (spastic paraplegia 20) gene encodes a multifunctional Spartin protein. SPG20 protein is highly expressed in adipose tissue and may be implicated in endosomal trafficking and microtubule dynamics. SPG20 is mutated in Troyer syndrome, a hereditary spastic paraplegia defined by degeneration of upper motor neurons, recent study showed that regulation of SPG20 on mitochondrial calcium homeostasis may contribute to the pathophysiology of Troyer syndrome. Hypermethylation of SPG20 promoter, found in colorectal cancer patients, may play a role in cytokinesis arrest in colorectal tumorigenesis.

Protokolle

Produktspezifische Protokolle
IF protocol for CL Plus 647 Spartin, SPG20 antibody CL647-13791Protokoll herunterladen
FC protocol for CL Plus 647 Spartin, SPG20 antibody CL647-13791Protokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen