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  • KD/KO Validated

Sortilin Polyklonaler Antikörper

Sortilin Polyklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF, FC (Intra)

Konjugation

CoraLite® Plus 647 Fluorescent Dye

Kat-Nr. : CL647-12369

Synonyme

100 kDa NT receptor, Glycoprotein 95, Gp95, Neurotensin receptor 3, NT3, NTR3, SORT1, Sortilin, sortilin 1

Galerie der Validierungsdaten

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  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CoraLite® Plus 647-conjugated Sortilin Polyclonal (CL647-12369)

    IF Staining of mouse brain using CL647-12369

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 647 Sortilin antibody (CL647-12369) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CoraLite® Plus 647-conjugated Sortilin Polyclonal (CL647-12369)

    IF Staining of mouse brain using CL647-12369

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 647 Sortilin antibody (CL647-12369) at dilution of 1:200.

  • Flow cytometry (FC) experiment of Neuro-2a cells using CoraLite® Plus 647-conjugated Sortilin Polyclonal (CL647-12369)

    FC experiment of Neuro-2a using CL647-12369

    1X10^6 Neuro-2a cells were intracellularly stained with 0.4 ug CoraLite® Plus 647 Anti-Human Sortilin (CL647-12369) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Geprüfte Anwendungen

Erfolgreiche Detektion in IFMaushirngewebe
Erfolgreiche Detektion in FCNeuro-2a-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Produktinformation

CL647-12369 bindet in IF, FC (Intra) Sortilin und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Sortilin fusion protein Ag2997
Vollständiger Name sortilin 1
Berechnetes Molekulargewicht 831 aa, 92 kDa
Beobachtetes Molekulargewicht 100-110 kDa
GenBank-ZugangsnummerBC023542
Gene symbol SORT1
Gene ID (NCBI) 6272
Konjugation CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Sortilin 1 (SORT1), is a trans-Golgi network (TGN) transmembrane protein and is a member of the Vps10p domain receptor family. SORT1 binds a number of unrelated ligands that participate in a wide range of cellular processes. It functions as a sorting receptor in the Golgi compartment and as a clearance receptor on the cell surface.

Protokolle

Produktspezifische Protokolle
IF protocol for CL Plus 647 Sortilin antibody CL647-12369Protokoll herunterladen
FC protocol for CL Plus 647 Sortilin antibody CL647-12369Protokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen