SQLE Monoklonaler Antikörper

SQLE Monoklonal Antikörper für FC (Intra)

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Hausschwein, human, Ratte

Anwendung

FC (Intra)

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

1C9A2

Kat-Nr. : CL594-67206

Synonyme

ERG1, FLJ30795, SE, SQLE, squalene epoxidase, Squalene monooxygenase

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Galerie der Validierungsdaten

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  FC experiment of HepG2 using CL594-67206

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human SQLE (CL594-67206, Clone:1C9A2) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL594-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

Anwendung	Verdünnung
Sample-dependent, check data in validation data gallery

Produktinformation

CL594-67206 bindet in FC (Intra) SQLE und zeigt Reaktivität mit Hausschwein, human, Ratten

• Getestete Reaktivität: Hausschwein, human, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SQLE fusion protein Ag3266
• Vollständiger Name: squalene epoxidase
• Berechnetes Molekulargewicht: 574 aa, 64 kDa
• Beobachtetes Molekulargewicht: 50-64 kDa
• GenBank-Zugangsnummer: BC017033
• Gene symbol: SQLE
• Gene ID (NCBI): 6713
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SQLE, also named as ERG1, SE and SM, belongs to the squalene monooxygenase family. It catalyzes the first oxygenation step in cholesterol synthesis, acting on squalene before cyclization into the basic steroid structure. SQLE may serve as a flux-controlling enzyme beyond 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGR, considered as rate limiting). It is also posttranslationally regulated by cholesterol-dependent proteasomal degradation. SQLE is subject to feedback regulation via cholesterol-induced degradation, which depends on its lipid-sensing N terminal regulatory domain. Truncation of SQLE occurs during its endoplasmic reticulum-associated degradation and requires the proteasome, which partially degrades the SQLE N-terminus and eliminates cholesterol-sensing elements within this region. The MW of SQLE is about 50-64 kDa. (PMID:21356516, PMID: 28972164)