SLP76 Monoklonaler Antikörper

SLP76 Monoklonal Antikörper für FC (Intra)

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

FC (Intra)

Konjugation

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

4H10B1

Kat-Nr. : CL647-66465

Synonyme

LCP2, Lymphocyte cytosolic protein 2, SLP 76, SLP 76 tyrosine phosphoprotein, SLP76

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Galerie der Validierungsdaten

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  FC experiment of Jurkat using CL647-66465

  1X10^6 Jurkat cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human SLP76 (CL647-66465, Clone:4H10B1) (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Jurkat-Zellen

Empfohlene Verdünnung

Anwendung	Verdünnung
Sample-dependent, check data in validation data gallery

Produktinformation

CL647-66465 bindet in FC (Intra) SLP76 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SLP76 fusion protein Ag3432
• Vollständiger Name: lymphocyte cytosolic protein 2 (SH2 domain containing leukocyte protein of 76kDa)
• Berechnetes Molekulargewicht: 533 aa, 60 kDa
• Beobachtetes Molekulargewicht: 76 kDa
• GenBank-Zugangsnummer: BC016618
• Gene symbol: LCP2
• Gene ID (NCBI): 3937
• Konjugation: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LCP2, also named as SLP76, is an adapter protein that acts as a substrate of the T cell antigen receptor (TCR)-activated protein tyrosine kinase pathway. It contains an N-terminal leucine Zip motif, a tyrosine-rich domain, a proline-rich domain and a C-terminal SH2 domain. It is constitutively associated with the growth factor receptor-bound protein 2 (Grb2)-related adapter protein 2 (Grap2, a.k.a. GADS) through the interaction of its proline residues with one of the SH3 domains of Grap2. SLP76 associates with growth factor receptor bound protein 2 (Grap2), and is thought to play a role TCR-mediated intracellular signal transduction. SLP76 is also expressed by mast cells, and mast cell activation was markedly impaired in SLP76-deficient mice.

Verwandte Produkte

Flow Cytometry Perm Buffer (10X)

PF00011-C