RALYL Monoklonaler Antikörper

RALYL Monoklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

IF, FC (Intra)

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

1F3F4

Kat-Nr. : CL594-67534

Synonyme

hnRNP core protein C like 3, HNRPCL3, hRALYL, RALY RNA binding protein like, RALYL, RNA binding Raly like protein

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Galerie der Validierungsdaten

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  IF Staining of HepG2 using CL594-67534

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594 RALYL antibody (CL594-67534, Clone: 1F3F4 ) at dilution of 1:200.

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  IF Staining of HepG2 using CL594-67534

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594 RALYL antibody (CL594-67534, Clone: 1F3F4 ) at dilution of 1:100.

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  FC experiment of HepG2 using CL594-67534

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human RALYL (CL594-67534, Clone:1F3F4) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: HepG2-Zellen
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Produktinformation

CL594-67534 bindet in IF, FC (Intra) RALYL und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: RALYL fusion protein Ag11676
• Vollständiger Name: RALY RNA binding protein-like
• Berechnetes Molekulargewicht: 291 aa, 32 kDa
• Beobachtetes Molekulargewicht: 35-40 kDa
• GenBank-Zugangsnummer: BC031090
• Gene symbol: RALYL
• Gene ID (NCBI): 138046
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RALY RNA binding protein-like (RALYL) belongs to the RALY subfamily. RALYL locates in 8q21.2, the function of which is RNA-binding and nucleotide binding. The RALYL high expression in the normal adrenal, kidney and brain (Shyamsundar et al., 2005). Furthermore, RALYL expression down-regulated correlates with mental disorder (Lee et al., 2007), Parkinson's disease (Moran et al., 2006), brain cancer (Maris et al., 2008), adrenal cancer (Giordano et al., 2009) and kidney cancer (Higgins et al., 2003). The expression of RALYL was significantly low in Clear Cell Renal Cell Carcinoma and correlated with a poor prognosis in a large number of clinical samples. Our findings showed that RALYL may be a potential therapeutic target as well as a poor prognostic