RAB27B Monoklonaler Antikörper

RAB27B Monoklonal Antikörper für FC (Intra)

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

FC (Intra)

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

2B9G9

Kat-Nr. : CL594-66944

Synonyme

C25KG, RAB27B, Ras related protein Rab 27B

---

Galerie der Validierungsdaten

• 

  FC experiment of A431 using CL594-66944

  1X10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human RAB27B (CL594-66944, Clone:2B9G9) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL594-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: A431-Zellen

Empfohlene Verdünnung

Anwendung	Verdünnung
Sample-dependent, check data in validation data gallery

Produktinformation

CL594-66944 bindet in FC (Intra) RAB27B und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: RAB27B fusion protein Ag18756
• Vollständiger Name: RAB27B, member RAS oncogene family
• Berechnetes Molekulargewicht: 218 aa, 25 kDa
• GenBank-Zugangsnummer: BC027474
• Gene symbol: RAB27B
• Gene ID (NCBI): 5874
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The Rab27 GTPase subfamily consists of two closely related homologs, Rab27a and Rab27b. Northern blot analysis revealed that Rab27b is expressed significantly only in testis, in which the predominant transcript was 1.4 kb in size. An 8-kb mRNA of unknown origin was detected in many tissues. Rab27b is associated with tubulovesicle membranes in the parietal cell and Rab27b may play a role in stimulation-associated membrane recruitment and gastric acid secretion. Rab27b is recently reported as a marker for breast cancer progression. It regulates invasive growth and metastasis in ER-positive breast cancer cell lines, and increased expression is associated with poor prognosis in humans.