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Phospho-P53 (Ser15) Rekombinanter Antikörper

Phospho-P53 (Ser15) Rekombinant Antikörper für FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC (Intra)

Konjugation

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

2J21

Kat-Nr. : CL647-80195

Synonyme

Antigen NY CO 13, Cellular tumor antigen p53, FLJ92943, LFS1, P53, Phosphoprotein p53, TP53, TRP53, tumor protein p53, Tumor suppressor p53

Galerie der Validierungsdaten

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  • Flow cytometry (FC) experiment of A431 cells using CoraLite® Plus 647-conjugated Phospho-P53 (Ser15) (CL647-80195)

    FC experiment of A431 using CL647-80195

    1X10^6 A431 cells untreated (dashed line) or UV treated A431 cells (red) were intracellularly stained with 0.5 ug CoraLite® Plus 647 Anti-Human Phospho-P53 (Ser15) (CL647-80195, Clone:2J21), or 0.5 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

Geprüfte Anwendungen

Erfolgreiche Detektion in FCmit UV behandelte A431-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Sample-dependent, check data in validation data gallery

Produktinformation

CL647-80195 bindet in FC (Intra) Phospho-P53 (Ser15) und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen Peptid
Vollständiger Name tumor protein p53
Berechnetes Molekulargewicht 44 kDa
Beobachtetes Molekulargewicht 53 kDa
GenBank-ZugangsnummerBC003596
Gene symbol TP53
Gene ID (NCBI) 7157
Konjugation CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

P53 is a 53 kDa protein that is activated in response to alteration of normal cell homeostasis, including DNA damage, nutrient starvation, heat shock, virus infection, pH change, hypoxia, and oncogene activation. P53 maintains genetic stability by regulating different processes, such as cell-cycle arrest, DNA synthesis and repair, programmed cell death, and energy metabolism. In non-stressed conditions these proteins bind p53, ubiquitylate it and target it for degradation by the proteasome. In stressed conditions the function of the MdM2-MdM4 complex is blocked by phosphorylation, protein-binding events and/or enhanced degradation. (PMID: 19935675, PMID: 24379683)