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Phospho-MST1 (Thr183)/MST2 (Thr180) Rekombinanter Antikörper

Phospho-MST1 (Thr183)/MST2 (Thr180) Rekombinant Antikörper für FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC (Intra)

Konjugation

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

1P6

Kat-Nr. : CL647-80093

Synonyme

DKFZp686A2068, KRS2, MST 1, MST1, serine/threonine kinase 4, STE20 like kinase MST1, STK4, YSK3

Galerie der Validierungsdaten

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  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 647-conjugated Phospho-MST1 (Thr183 (CL647-80093)

    FC experiment of HeLa using CL647-80093

    1X10^6 HeLa cells untreated (dashed lines) or treated with Calyculin A (red) were intracellularly stained with 0.25 ug CoraLite® Plus 647 Anti-Human Phospho-MST1 (Thr183)/MST2 (Thr180) (CL647-80093, Clone:1P6), or 0.25 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

Geprüfte Anwendungen

Erfolgreiche Detektion in FCMit Calyculin A behandelte HeLa-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Sample-dependent, check data in validation data gallery

Produktinformation

CL647-80093 bindet in FC (Intra) Phospho-MST1 (Thr183)/MST2 (Thr180) und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen Peptid
Vollständiger Name serine/threonine kinase 4
Berechnetes Molekulargewicht 56 kDa
Beobachtetes Molekulargewicht 59 kDa
GenBank-ZugangsnummerBC005231
Gene symbol STK4
Gene ID (NCBI) 6789
Konjugation CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Mammalian STE20-like serine-threonine kinase MST1, encoded by the STK4 gene, is a multifunctional protein. MST1 and its closest paralogs MST2 (encoded by the STK3 gene), MST3, and MST4 are members of the Class II Germinal Center Family of Protein Kinases . STK3/4 and LATS1/2 (large tumor suppressor 1 and 2) are core kinase components of the Hippo tumor suppressor pathway in mammalians . In the conventional Hippo pathway, the STK3/4 and LATS1/2 signaling cascade phosphorylates and inactivates the transcriptional coactivator YAP1 (yes associated protein 1) and its close paralog WWTR1]. YAP1 and WWTR1 do not have DNA binding domains and they exert their biological outputs, such as cell proliferation and survival, by interacting with the TEAD1-4 transcription factors. Lines of evidence have indicated that dysregulation or loss of STK4/Hippo signaling is linked to developmental disorders and carcinogenesis with poor prognosis. STK4 is a stress-induced kinase and it can be activated in response to cell-death inducers. Autophosphorylation of STK4 at Thr183 (Thr180 in STK3) in the activation loop is a key activation mechanism for STK4/3 because phosphorylation of Thr183/180 causes the cleavage of STK4 by caspases under apoptotic conditions. The caspase-cleavage results in a more active STK4 protein (STK4-N, an amino-terminally truncated STK4), which localizes into the nucleus and induces apoptosis through histone modifications and chromatin condensations.