• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Phospho-Hamartin/TSC1 (Ser511) Rekombinanter Antikörper

Phospho-Hamartin/TSC1 (Ser511) Rekombinant Antikörper für FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

FC (Intra)

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

3N6

Kat-Nr. : CL594-80340

Synonyme

Hamartin, KIAA0243, LAM, TSC, TSC1, tuberous sclerosis 1, Tuberous sclerosis 1 protein

Galerie der Validierungsdaten

Filter:
  • Flow cytometry (FC) experiment of HeLa cells using CoraLite®594-conjugated Phospho-Hamartin/TSC1 (Ser (CL594-80340)

    FC experiment of HeLa using CL594-80340

    1X10^6 HeLa cells untreated (left) or treated with Calyculin A (right) were intracellularly stained with 0.5 ug CoraLite®594 Anti-Human Phospho-Hamartin/TSC1 (Ser511) (CL594-80340, Clone:3N6) (red), or 0.5 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 50% MeOH.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite®594-conjugated Phospho-Hamartin/TSC1 (Ser (CL594-80340)

    FC experiment of HeLa using CL594-80340

    1X10^6 HeLa cells untreated (dashed lines) or treated with Calyculin A (red) were intracellularly stained with 0.5 ug CoraLite®594 Anti-Human Phospho-Hamartin/TSC1 (Ser511) (CL594-80340, Clone:3N6) (red), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with 50% MeOH.

Geprüfte Anwendungen

Erfolgreiche Detektion in FCMit Calyculin A behandelte HeLa-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Sample-dependent, check data in validation data gallery

Produktinformation

CL594-80340 bindet in FC (Intra) Phospho-Hamartin/TSC1 (Ser511) und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen Peptid
Vollständiger Name tuberous sclerosis 1
Berechnetes Molekulargewicht 130 kDa
Beobachtetes Molekulargewicht150 kDa
GenBank-ZugangsnummerNM_000368
Gene symbol TSC1
Gene ID (NCBI) 7248
Konjugation CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TSC1, also named as Hamartin, is a tumor suppressor gene syndrome whose manifestations can include seizures, mental retardation, autism, and tumors in the brain, retina, kidney, heart, and skin. The tuberous sclerosis 1 (TSC1)/TSC2 tumor suppressor complex serves as a repressor of the mTOR pathway, and disruption of TSC1/TSC2 complex function may contribute to tumorigenesis. TSC1 is phosphorylated by IKKb at Ser487 and Ser511 in vitro and in vivo, and phosphorylation at these sites enhances dissociation of the TSC1/TSC2 complex, which in turn induces mTOR activation. (PMID: 17693255). TSC1 is also threonine-phosphorylated at three sites (Thr417, Ser584, and Thr1047) in a reaction catalyzed by cyclin-dependent kinase 1 (CDK1) (PMID: 14551205).