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Phospho-AKT (Ser473) Rekombinanter Antikörper

Phospho-AKT (Ser473) Rekombinant Antikörper für FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC (Intra)

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

2E17

Kat-Nr. : CL594-80455

Synonyme

AKT, AKT1, PKB, PKB ALPHA, PRKBA, Protein kinase B, Proto oncogene c Akt, RAC, RAC ALPHA, RAC PK alpha

Galerie der Validierungsdaten

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  • Flow cytometry (FC) experiment of HEK-293 cells using CoraLite®594-conjugated Phospho-AKT (Ser473) Recom (CL594-80455)

    FC experiment of HEK-293 using CL594-80455

    1X10^6 HEK-293 cells untreated (dashed lines) or treated with Calyculin A (red) were intracellularly stained with 0.13 ug CoraLite®594 Anti-Human Phospho-AKT (Ser473) (CL594-80455, Clone:2E17) , or 0.13 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 80% MeOH.

  • Flow cytometry (FC) experiment of HEK-293 cells using CoraLite®594-conjugated Phospho-AKT (Ser473) Recom (CL594-80455)

    FC experiment of HEK-293 using CL594-80455

    1X10^6 HEK-293 cells untreated (left) or treated with Calyculin A (right) were intracellularly stained with 0.13 ug CoraLite®594 Anti-Human Phospho-AKT (Ser473) (CL594-80455, Clone:2E17), or 0.13 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with 80% MeOH.

Geprüfte Anwendungen

Erfolgreiche Detektion in FCMit Calyculin A behandelte HEK-293-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Sample-dependent, check data in validation data gallery

Produktinformation

CL594-80455 bindet in FC (Intra) Phospho-AKT (Ser473) und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen Peptid
Vollständiger Name v-akt murine thymoma viral oncogene homolog 1
Beobachtetes Molekulargewicht 58 kDa
GenBank-ZugangsnummerNM_005163
Gene symbol AKT1
Gene ID (NCBI) 207
Konjugation CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AKT is a serine/threonine kinase and it participates in the key role of the PI3K signaling pathway. Phosphatidylinositol-3 kinase (PI3K) is the key regulator of AKT activation. The recruitment of inactive AKT protein to PIP3-rich areas of the plasma membrane results in a conformational change that exposes the activation loop of AKT. AKT's activating kinase, phosphoinositide-dependent protein kinase (PDK1) , is also recruited to PIP3 microdomains. PDK1 phosphorylates AKT on threonine 308 (Thr308) of the exposed activation loop, activating AKT and leading to a second phosphorylation of AKT at serine 473 (Ser473) by a kinase presumed to be mTORC2 that further potentiates kinase activity. Active AKT will phosphorylate various downstream protein targets that control cell growth and translational control and act to suppress apoptosis. (PMID: 31594388, PMID: 30808672)