PNPLA3 Monoklonaler Antikörper
PNPLA3 Monoklonal Antikörper für IF
Wirt / Isotyp
Maus / IgG1
Getestete Reaktivität
human, Ratte
Anwendung
IF
Konjugation
CoraLite®594 Fluorescent Dye
CloneNo.
1D2B11
Kat-Nr. : CL594-67369
Synonyme
Galerie der Validierungsdaten
Geprüfte Anwendungen
Erfolgreiche Detektion in IF | HeLa-Zellen |
Empfohlene Verdünnung
Anwendung | Verdünnung |
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Immunfluoreszenz (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Produktinformation
CL594-67369 bindet in IF PNPLA3 und zeigt Reaktivität mit human, Ratten
Getestete Reaktivität | human, Ratte |
Wirt / Isotyp | Maus / IgG1 |
Klonalität | Monoklonal |
Typ | Antikörper |
Immunogen | PNPLA3 fusion protein Ag27407 |
Vollständiger Name | patatin-like phospholipase domain containing 3 |
Beobachtetes Molekulargewicht | 53 kDa |
GenBank-Zugangsnummer | BC065195 |
Gene symbol | PNPLA3 |
Gene ID (NCBI) | 80339 |
Konjugation | CoraLite®594 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 588 nm / 604 nm |
Form | Liquid |
Reinigungsmethode | Protein-G-Reinigung |
Lagerungspuffer | BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3. |
Lagerungsbedingungen | Bei -20°C lagern. Vor Licht schützen. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
PNPLA3 belongs to a family of proteins that share a domain that was first identified in patatin, a major soluble protein of potato tubers with nonspecific acyl hydrolase activity. The domain differs from classical lipases by employing a catalytic dyad (Ser-Asp), rather than a catalytic triad, to effect hydrolysis. PNPLA3 is expressed primarily in liver and adipose tissue, in which it partitions to membranes and lipid droplets. Real-time PCR of cDNA from human tissues indicated that PNPLA3 expression was highest in the liver, followed by skin and adipose tissue. PNPLA3 is expressed at the highest levels in adipose tissue of mice. The PNPLA3 protein was expected at 50-70 kDa, as observed; the additional band at approximately 130-150 kDa is a oligomer bands. (PMID: 20385813, PMID: 24931521, PMID: 23023705)
Protokolle
Produktspezifische Protokolle | |
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IF protocol for CL594 PNPLA3 antibody CL594-67369 | Protokoll herunterladen |
Standard-Protokolle | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |