PGC1a Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

PGC1a Monoklonal Antikörper für FC (Intra)

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

FC (Intra)

Konjugation

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

1C1B2

Kat-Nr. : CL647-66369

Synonyme

LEM6, Ligand effect modulator 6, PGC 1 alpha, PGC 1(alpha), PGC 1v, PGC1, PGC1A, PPAR gamma coactivator 1 alpha, PPARGC 1 alpha, PPARGC1, PPARGC1A

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Galerie der Validierungsdaten

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  FC experiment of HeLa using CL647-66369

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human PGC1a (CL647-66369, Clone:1C1B2) (red), or 0.2 ug APC-65128 ; APC Mouse IgG2b Isotype Control (APC-65128, Clone: MPC-11) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

Anwendung	Verdünnung
Sample-dependent, check data in validation data gallery

Produktinformation

CL647-66369 bindet in FC (Intra) PGC1a und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: peroxisome proliferator-activated receptor gamma, coactivator 1 alpha
• Berechnetes Molekulargewicht: 91 kDa
• Beobachtetes Molekulargewicht: 100 kDa
• GenBank-Zugangsnummer: NM_013261
• Gene symbol: PPARGC1A
• Gene ID (NCBI): 10891
• Konjugation: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PPARGC1A, also named as Peroxisome proliferator-activated receptor gamma coactivator 1-alpha, is a 798 amino acid protein, which Contains 1 RRM (RNA recognition motif) domain and localizes in the nucleus. PPARGC1A is transcriptional coactivator for steroid receptors and nuclear receptors. PPARGC1A can regulate key mitochondrial genes that contribute to the program of adaptive thermogenesis and plays an essential role in metabolic reprogramming in response to dietary availability through coordination of the expression of a wide array of genes involved in glucose and fatty acid metabolism. PPARGC1A exists an isoform in the liver and the molecular weight of it is 77 kDa. Except for a deletion of 127 amino acids at the N terminus, the protein, termed L-PGC-1 α, is identical to PGC-1.