NK1.1 (CD161) Monoklonaler Antikörper

NK1.1 (CD161) Monoklonal Antikörper für FC

Wirt / Isotyp

Maus / IgG2a, kappa

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

PK136

Kat-Nr. : CL647-65138

Synonyme

CD161, Klrb1a, Ly55a, NK1.1, NK1.1 (CD161), NKR P1A, Nkrp1 a, NKRP12, Nkrp1a

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Galerie der Validierungsdaten

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  FC experiment of mouse splenocytes using CL647-65138

  1X10^6 C57BL/6 mouse splenocytes were surface stained with FITC Anti-Mouse CD49b (DX5) (FITC-65065, Clone: DX5) and CoraLite® Plus 647-conjugated Mouse IgG2a isotype control antibody (left) or 5.00 ul CoraLite® Plus 647-conjugated Anti-Mouse NK1.1 (CD161) (CL647-65138, Clone: PK136) (right). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Maus-Splenozyten

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

CL647-65138 bindet in FC NK1.1 (CD161) und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Maus / IgG2a, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: NK-1+-Zellen aus Mausmilz und -knochenmark
• Vollständiger Name: killer cell lectin-like receptor subfamily B member 1A
• GenBank-Zugangsnummer: BC120707
• Gene symbol: Klrb1a
• Gene ID (NCBI): 17057
• Konjugation: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS mit 0,1% Natriumazid und 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NK1.1 (CD161), also known as KLRB1 or NKR-P1A, is a type II transmembrane C-type lectin-like receptor and is expressed on the cell membrane as disulfide-linked homodimer (PMID: 8077657). It is expressed by the majority of NK cells and subsets of peripheral T cells, including both CD4+ and CD8+ T cells, and is expressed preferentially on adult T cells with a "memory" antigenic phenotype (PMID: 8077657; 22566826). Expression of CD161 correlates with the cytotoxic function of CD16+ NK cells, and ligation of CD161 with its ligand LLT1 inhibits NK cell cytotoxicity and cytokine secretion (PMID: 29686665).