- Featured Product
- KD/KO Validated
NANOG Polyklonaler Antikörper
NANOG Polyklonal Antikörper für FC (Intra)
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus, Ratte
Anwendung
FC (Intra)
Konjugation
CoraLite® Plus 647 Fluorescent Dye
Kat-Nr. : CL647-14295
Synonyme
Galerie der Validierungsdaten
Geprüfte Anwendungen
Erfolgreiche Detektion in FC | NCCIT-Zellen |
Empfohlene Verdünnung
Anwendung | Verdünnung |
---|---|
Sample-dependent, check data in validation data gallery |
Produktinformation
CL647-14295 bindet in FC (Intra) NANOG und zeigt Reaktivität mit human, Maus, Ratten
Getestete Reaktivität | human, Maus, Ratte |
Wirt / Isotyp | Kaninchen / IgG |
Klonalität | Polyklonal |
Typ | Antikörper |
Immunogen | NANOG fusion protein Ag5645 |
Vollständiger Name | Nanog homeobox |
Berechnetes Molekulargewicht | 35 kDa |
Beobachtetes Molekulargewicht | 35-40 kDa |
GenBank-Zugangsnummer | BC160187 |
Gene symbol | NANOG |
Gene ID (NCBI) | 79923 |
Konjugation | CoraLite® Plus 647 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 654 nm / 674 nm |
Form | Liquid |
Reinigungsmethode | Antigen-Affinitätsreinigung |
Lagerungspuffer | BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3. |
Lagerungsbedingungen | Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
Nanog is a member of the homeobox family of DNA binding transcription factors and has been shown to maintain embryonic stem (ES) cell self-renewal independently of leukemia inhibitory factor (LIF)/Stat3. Nanog mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Functionally, Nanog works together with other key pluripotent factors (Oct4, Sox2, and Lin28) to reprogram human fibroblasts and generate induced pluripotent stem (iPS) cells. These key factors form a regulatory network to support or limit each other's expression level, which maintains the properties of ES cells. Affinity purified rabbit anti-Nanog can be used to demonstrate pluripotency of ES and IPS cells. There are two kinds of variants could recognized by NANOG, one is normal form (~39kd), the other is post-translation modified form (~48kd) (21136380 ). Nanog exists two isoforms with molecular weight 34.4 kDa and 31.9 kDa. (PMID: 21969378)
Protokolle
Produktspezifische Protokolle | |
---|---|
FC protocol for CL Plus 647 NANOG antibody CL647-14295 | Protokoll herunterladen |
Standard-Protokolle | |
---|---|
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |