MYC tag Monoklonaler Antikörper

MYC tag Monoklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

rekombinanten Protein

Anwendung

IF, FC (Intra)

Konjugation

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

1A5A2

Kat-Nr. : CL647-60003

Synonyme

---

Galerie der Validierungsdaten

• 

  IF Staining of Transfected HEK-293 using CL647-60003

  Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using CoraLite® Plus 647-conjugated MYC tag antibody (CL647-60003, Clone: 1A5A2 ) at dilution of 1:800.

• 

  FC experiment of Transfected HEK-293 using CL647-60003

  1X10^6 Transfected HEK-293 cells were intracellularly stained with 0.4 ug CoraLite® Plus 647 Anti-N/A MYC tag (CL647-60003, Clone:1A5A2) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  FC experiment of Transfected HEK-293 using CL647-60003

  1X10^6 Transfected HEK-293 cells were stained with 0.2 ug CoraLite® Plus 647-conjugated Anti-N/A MYC tag (CL647-60003, Clone:1A5A2) (blue), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH.

• 

  FC experiment of Transfected HEK-293 using CL647-60003

  1X10^6 Transfected HEK-293 cells were stained with 0.2 ug CoraLite® Plus 647-conjugated Anti-N/A MYC tag (CL647-60003, Clone:1A5A2) (blue), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: Transfizierte HEK-293-Zellen
• Erfolgreiche Detektion in FC: Transfizierte HEK-293-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:400-1:1600

Sample-dependent, check data in validation data gallery

Produktinformation

CL647-60003 bindet in IF, FC (Intra) MYC tag und zeigt Reaktivität mit rekombinanten Protein

• Getestete Reaktivität: rekombinanten Protein
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: MYC tag
• Konjugation: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The c-Myc tag corresponds to amino acid residues(EQKLISEEDL) of the human c-Myc protein. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. Myc-Tag mouse mAb detects recombinant proteins containing the Myc tag. The antibody recognizes the Myc-tag EQKLISEEDL fused to either the amino- or carboxy- terminus of targeted proteins.

Verwandte Produkte

Flow Cytometry Perm Buffer (10X)

PF00011-C